反向LncRNA靶基因预测服务

服务内容：
与常规的从lncRNA出发预测靶基因不同，反向long noncodingRNA(lncRNA)靶基因预测是从感兴趣的已知基因开始（例如p53，COX-2等与肿瘤等密切相关的基因，这些基因可以来自芯片分析结果、NLP结果或客户给定的任何基因列表），利用我们的longnoncoding RNA(lncRNA)数据库和靶基因预测软件pipeline，找到潜在的调节这些基因的lncRNA。

研究方法：
我们的服务提供：

1.获取感兴趣的基因集合

这些基因可以来自芯片分析结果、NLP结果或客户给定的任何基因列表。如p53，COX-2。

2.建立lncRNA数据库

我们通过整合已知所有lncRNA数据库，形成一个全面的包含所有lncRNA序列的数据库

3.细胞组织特异高表达lncRNA的鉴定（可选）

根据客户提供的细胞组织信息，我们搜索已有的tiling芯片以及新一代测序得到的转录组信息，从全基因组已知lncRNA的中鉴定高表达的lncRNA。

4.cis靶基因预测

Cis作用[5]是lncRNA的一种靶基因作用方式。我们利用基因组注释和基因组浏览器鉴定lncRNA的可能的靶基因。一般在启动子区域同向转录的靶基因一般是促进表达作用，反向为抑制。而在3’-UTR区域时，部分情况下反向也为促进表达，见[14]。

5.trans靶基因预测

trans作用是lncRNA的另一种靶基因作用方式。我们利用RNAplex（http://www.tbi.univie.ac.at/~htafer/）等鉴定lncRNA的可能的靶基因，一个例子请见[15]。
综上，一个可能分析流程如下：



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