荧光定量PCR服务要求
1. 请您提供新鲜的且尽量多的材料（各种材料的 RNA 提取量请参照“总 RNA 的提取”），或直接提供纯化好的 总 RNA （大于 5 ug/ 样品）；（ 各种材料的 DNA 提取量请参照“ D NA 的提取”），或直接提供纯化好的 D NA （大于 5 ug/ 样品 ) 。
2. 请通过 E-mail 提供已知的全长基因序列。
3. 请提供尽可能详细的背景资料： DNA/ RNA 来源、丰度等
荧光定量PCR操作程序
1. 根据基因序列设计特异性的引物和荧光探针。
2. 提取 DNA/ RNA 。
3. Real Time PCR(荧光定量PCR)，进行实验结果分析
4. 实验完成后，提供完整的实验报告（含软件分析结果）及引物等实验材料
荧光定量PCR收费标准
1、RNA提取：10－20个样品的RNA提取是：150元/个；20－30个样品的RNA提取是：120 元/个；30个以上样品的RNA提取是：100元/个；每份材料最少应提供：100mg/样品
2、一个指标每个样品为150元；二个指标每个样品为240元；三指标每个样品为300元，三个指标以上每个样品为80元/反应。
3、引物合成为每个碱基2.1元，探针标记为1200元/条，如果是用sybr green I(染料)做，则免去探针的费用，染料赠送。
4、内参的GAPDH引物探针免费，标准曲线免费赠送。
5、不再收取任何的基础费或者开机费。
(如果样本或者指标数更多，再面议)，5000.00元以内的服务费，一律100%预付；
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重点品牌
RNA 干扰 Genetools 、Chemicon 、 Santa Cruz
信号转导研究 Genetools 、Chemicon 、CST 、Santa Cruz
分子生物学 Toyobo 、Promega 、Strategene、Bio-Rad
干细胞、细胞凋亡 Chemicon 、R&D 、Santa Cruz 、Biovision
细胞因子及检测 Chemicon 、R&D 、Peprotech 、Prospec
抗体与标记抗体 Santa Cruz 、 R&D 、 Abnova、Cayman
细胞培养及药筛 Clark、Cisbio、Hyclone 、Gibco、Chemicon
PCR与定量PCR Bio-Rad 、 Stratagene 、Promega 、Toyobo
蛋白质研究 Pierce 、 Pharmacia 、 Pannomics 、 Bio-Rad
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