在培养脊髓运动神经元过程中所形成由神经元和网络活动构成的网络

这是第一次报告多电极记录运动神经元网络。从E15大鼠脊髓腹角分离出来的神经细胞放在Med-64探针里培养。大多数培养的神经元具有神经丝、胆碱乙酰转移酶和Hb9 等运动神经元的特点。运动神经元网络的活动特点是具有单个细胞尖峰，并且是自发的。尖峰和网络突发是由γ-氨基丁酸拮抗剂 (GABA)和乙酰胆碱刺激引起的，也能被GABA和谷氨酸受体拮抗剂本身所抑制。培养的胚胎运动神经元网络是一个研究运动神经元发展、运动神经疾病生理及病理机制的理想模型系统。

Neuronal and network activity in networks of cultured spinal motor neurons.

Zhang HM, Robinson N, Gómez-Curet I, Wang W, Harrington MA.

Department of Biological Sciences, Delaware State University, Dover, DE 19901, USA.

This is the first report of multielectrode recordings from networks of cultured motor neurons. Neurons isolated from the ventral horns of spinal cords of E15 rats were cultured on MED64 probes. The majority of the neurons in the cultures are positive for neurofilament, choline acetyltransferase, and Hb9, characteristics of motor neurons. The activity of the motor neuron network is characterized by spiking of individual cells as well as spontaneous, synchronized bursts involving all active electrodes. Both spiking and network bursts are stimulated by GABA antagonists and acetylcholine, and are inhibited by GABA itself and glutamate antagonists. Networks of cultured embryonic motor neurons make a good model system for studying motor neuron development and physiology as well as the pathophysiology of motor neuron disease.

文章来源：http://www.ncbi.nlm.nih.gov/pubmed/19436229?ordinalpos=1&itool=EnvportPanel.Pubmed_RVDocSum