过氧化氢蒸汽 ( H P V ) 生物有效性

过氧化氢蒸汽 ( H P V ) 生物有效性

©Bioquell UK Ltd (2011). 版权所有                技术报告HPV 生物有效性069/11

过氧化氢蒸汽（HPV）灭菌的特点是无残留（其分解产物只有水和氧气）和仅需较低的环境温度 ，蒸汽发生阶段增加了灭菌过程的实用性。过氧化氢蒸汽（HPV）已经对许多生物及不同级别的生物进行了灭菌测试，实验证明：过氧化氢蒸汽（HPV）对包括细菌、病毒和真菌在内的微生物均具有广谱杀菌的功效。过氧化氢蒸汽（HPV）对于普遍存在于环境表面的细菌孢子灭杀的有效性经验证是可以反复进行的，因此，它位于Spaulding分类中的第一位。文件中列出的有机体是按广义分类的（例如细菌、病毒和真菌）并按照它们的微生物特点进行了划分。实验证明，利用过氧化氢蒸汽（HPV）对微生物进行消毒灭菌可达到6-log的灭杀率。

目录

1 . 经测试的生物及参考文献/出处

1.1 细菌和细菌孢子

1.2 病毒 

1.3 噬菌体 

1.4 真菌

1.5 线虫类和原生动物

1.6 其他

2. 参考文献/出处 

 1.  经测试的生物及参考文献/ 出处

1.1 细菌和细菌孢子

1.2 病毒

1.3 噬菌体

1.4 真菌

1.5 线虫类和原生动物

1.6 其他

i 证据显示HPV可以灭活 蛋白质易传染粒子 (38).

i 重要研究显示HPV 对断裂的DNA在分子生物学应用中起到至关重要的作用(39).

 

 2.  参考文献/  出处

1. Rogers, J. V., C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki, K. B. Riggs, M. L. Taylor, and J. Ch a ng.

2005. Decontamination assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus

spores on indoor surfaces using a hydrogen peroxide gas generator. J . Appl.Microbiol. 99:739-748.

2. Bacillus anthracis (Anthrax) deactivation investigation. Defence Science and Technology

Laboratory (Dstl), Porton Down UK. 2002.

3. Kokubo, M., T. Inoue, and J. Akers. 1998. Resistance of common environmental spores

of the genus Bacillus to vapor hydrogen peroxide. PDA.J.Pharm.Sci.Technol. 52:228 - 231.

4. McDonnell, G., G. Grignol, and K. Antloga. 2002. Vapour-phase hydrogen peroxide decontamination of

food contact surfaces. Dairy.Food.Environ.Sanitat. 22:868-873.

5. Spaulding, E. H. 1972. Chemical disinfection and antisepsis in the hospital. J.Hosp.Res. 9:5 - 31.

6. Rickloff, J. R. and reliski, P. A. 1989. Resistance of various micro-organisms to vaporized hydrogen peroxide in

prototype tabletop sterilizer. 89 th General Meeting of the American Society for Microbiology (ASM). New

Orleans.

7. Cabinet bio-decontamination trial. March 1995. Health Protection Agency (previously

Centre for Applied Microbiology and Research), Porton Down UK. 1995.

8. US Environmental Protection Agency (EPA) registered steril a nt. EPA registration number

72372-1 - 86703. 2009.

9. Johnston, M. D., S. Lawson, and J. A. Otter. 2005. Evaluation of hydrogen peroxide vapour as a method for the

decontamination of surfaces contaminated with Clostridium botulinum spores. J.Microbiol.Methods. 60:403 - 411.

10. Boyce, J. M., N. L. Havill, J. A. Otter, L. C. McDonald, N. M. Adams, T. Cooper,

A. Thompson, L. Wiggs, G. Killgore, A. Tauman, and J. Noble - Wang. 2008. Impact of hydrogen peroxide

vapor room decont a mination on Clostridium dif f icile environmental contamination and transmission in a

healthcare setting. Infect.Control.Hosp.Epidemiol.

29:723-729.

11. Otter, J. A. and G. L. French. 2009. Survival of nosocomial bacteria and spores on surfaces and inactivation

by hydrogen peroxide vapor. J.Clin.Microbiol. 47:205 - 207.

12. Case study f rom a room bio-decontamination at Imperial College School of Medicine.

2003. Contact Bioquell for further details.

13. Hall, L., J. A. Otter, J. Chewins, and N. L. Wengenack. 2007. Use of hydrogen peroxide vapor for deactivation of

Mycobacterium tuberculosis in a biological safety cabinet and a room. J.Clin.Microbiol. 45:810 - 815.

14. French, G. L., J. A. Otter, K. P. Shannon, N. M. Adams, D. Watling, a nd M. J. Parks.

2004. Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus

(MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination.

J.Hosp.Infect. 57:31-37.

15. Otter, J. A., M. Cummins, F. Ahmad, van Tonder C., and Y. J. Drabu. 2007. Assessing the biological ef f icacy and

rate of recontamin a tion following hydrogen peroxide vapour decontamination. J.Hosp.Infect. 67:182-188.

16. Jeanes, A., G. Rao, M. Osman, and P. Merrick. 2005. Eradication of persistent environmental MRSA.

J.Hosp.Infect. 61:85-86.

17. Dryden, M., R. Parnaby, S. Dailly, T. Lewis, K. Davis - Blues, J. A. Otter, and A. M. Kearns.

2008. Hydrogen peroxide vapour decontamination in the control of a polyclonal meticillin- resistant Staphylococcus

aureus outbreak on a surgical ward. J.Hosp.Infect. 68:190-192.

18. Determination of the effectiveness of VPHP against methicillin-resistant Staphylococcus aureus,

Staphylococcus epidermidis and Bacillus stearothermophilus. Health Protection Agency (previously Centre for

Applied Microbiology and Research),

Porton Down UK. 2001.

19. Assessment of the ef f icacy of vapour phase hydrogen peroxide as a room disinfectant. Health Protection Agency

(previously Centre for Applied Microbiology and Research), Porton Down UK. 2003.

20. Otter, J. A., S. Yezli, M. A. Shouten, A. R. van Zanten, G. Houmes - Zielman, and M. Nohlmans-Paulssen.

2010. Hydrogen peroxide vapor (HPV) decontamin a tion of an intensive care unit to remove

environmental reservoirs of multidrug-resist a nt

Gram - negative rods during an outbreak. Am.J.Infect.Control, accepted.

21. Bates, C. J. and R. Pearse. 2005. Use of hydrogen peroxide vapour for environmental control during a Serratia

outbreak in a neonatal intensive care unit. J.Hosp.Infect.

61:364-366.

22. Rogers, J. V., W. R. Richter, M. Q. Shaw, a nd Y. W. Choi. 2008. Vapour - phase hydrogen peroxide inactivates

Yersinia pestis dried on polymers, steel, and glass surfaces. Lett.Appl.Microbiol. 47:279-285.

23. Otter, J. A., J. Chewins, D. Windsor, and H. Windsor. 2008. Microbiological contamination

in cell culture: a potential role for hydrogen peroxide vapour (HPV)? Cell.Biol.Int.

32:326-327.

24. Rickloff, J. R. 1990. Use of Vapourized Hydrogen Peroxide for the Bio-decontamination of

Enclosed Areas. Interphex USA Con f erence. New York.

25. Heckert, R. A., M. Best, L. T. Jordan, G. C. Dulac, D. L. Eddington, and W. G. Sterritt.

1997. E f ficacy of vaporized hydrogen peroxide against exotic animal viruses.

Appl.Environ.Microbiol. 63:3916-3918.

26. Adenovirus deactivation trials. Conducted in commercial confidence. 2003. Contact Bioquell for

further details.

27. Viral deactivation trials. Conducted in commercial confidence. 2002. Contact Bioquell

for further details.

28. McDonnell, G., Belete, B., Fritz, C., and Hartling, J. 2001. Room decontamination with vapour hydrogen

peroxide VHP for environmental control of parvovirus. American Association for Laboratory Animal Science(AALAS), Annual meeting. Baltimore, MD.

29. Rudnick, S. N., J. J. McDevitt, M. W. First, and J. D. Spengler. 2009. Inactivating

influenza viruses on surfaces using hydrogen peroxide or triethylene glycol at low vapor concentrations.

Am.J.Infect.Control. 37:813-819.

30. Investigation into the efficacy of hydrogen peroxide vapour in the bio-deactivation of Dengue virus. 2003.

Conducted in commercial confidence. Contact Bioquell for f urther details.

31. Otter, J. A., M. Barnicoat, J. Down, D. Smyth, S. Yezli, and A. Jeanes. 2010. Hydrogen peroxide vapor (HPV)

decontamination of an intensive care unit room used to treat a patient with Lassa fever. J.Hosp.Infect.in press.

32. Otter, J. A. and A. Budde-Niekiel. 2009. Hydrogen peroxide vapor: a novel method for the environmental

control of lactococcal bacteriophages. J.Food.Prot. 72:412 - 414.

33. Pottage, T., C. Richardson, S. Parks, J. T. Walker, and A. M. Bennett. 2010. Evaluation of hydrogen peroxide

gaseous disinfection systems to decontaminate viruses.

J.Hosp.Infect. 74:55 - 61.

34. Information supplied with kind permission of Eli Lilly and Company, Indianapolis, Indiana.

1996.

35. Hall, L., J. A. Otter, J. Chewins, and N. L. Wengenack. 2008. Deactivation of the dimorphic f ungi

Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis using hydrogen peroxide vapor.

Med.Mycol. 46:189-191.

36. Gustin, E. J., McDonnell, G. E., Mullen, G., and Gordon, B. E. 2002. The efficacy of vapour phase hydrogen

peroxide against nematode infestation: the Caenorhabditis ele g ans model. American Association for

Laboratory Animal Science (AALAS), Annual meeting. San Antonio, Texas, USA

37. Krause, J. and Riedesel, H. 2002. Elimination of pinworm eggs from caging equipment with vapourised

hydrogen peroxide. Report from the Max-Planck - Institute for experimental medicine. Association for

Laboratory Animal Science (AALAS) national meeting. San Antonio, Texas, USA

38. Fichet, G., K. Antloga, E. Comoy, J. P. Deslys, a nd G. McDonnell. 2007. Prion inactivation using a new gaseous

hydrogen peroxide sterilisation process. J.Hosp.Infect. 67:278 - 286.

39. DNA degradation studies. Conducted in commercial confidence. 2008. Contact Bioquell

for further details.

Bioquell公司的过氧化氢蒸汽（HPV）作为一种生物消毒剂因其对微生物具有广谱杀菌及快速灭活的功效已在全球各国家/地区得到广泛应用。