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Molecular Biology/分子生物学
Protein Production Systems and Purification /蛋白质表达系统以及纯化
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| 服务商： 上海美迪西生物医药有限公司 | 查看该公司所有服务 >> |
- Molecular Biology/分子生物学
- Plasmid DNA preparation/质粒制备
- Subcloning/亚克隆
- Custom ORF cloning/ORF克隆
- Mutagenesis services/基因突变
- Molecular diagnosis/分子诊断
- Protein Production Systems and Purification /蛋白质表达系统以及纯化
- The E. Coli System/大肠杆菌表达系统
○ Generation of Recombinant Plasmid /重组质粒构建
○ Expression Test in Host /重组表达测试
○ Soluble Expression Test / 可溶性表达条件测试
○ Small Scale Expression and Purification (2L Cell Culture)/小规模蛋白表 达 和纯化（2L培养基培养产物）
○ Scale Up Expression and Purification/大规模蛋白表达和纯化（10L以上）
- The Baculovirus System/昆虫细胞表达系统
○ 重组杆粒（bacmid DNA）制备/Generation of recombinant Bacmid DNA
○ 重组杆状病毒制备/Preparation of recombinant baculovirus
○ 重组病毒滴度测定/Titration of baculovirus
○ 重组蛋白表达条件优化/Protein expression verification and optimization
○ 小规模蛋白表达和纯化（500 ml培养物）/Small scale expression and purification of recombinant protein in insect cell
○ 大规模蛋白表达和纯化（可选）/Scale up expression and purification of recombinant protein in insect cell
- The Mammalian System/哺乳动物表达系统
- CHO, Vero, BHK, 293 cells etc/CHO, Vero, BHK, HEK293 等多种表达系统
- Adherence and suspension culture/贴壁及悬浮培养
- Transient expression screen/瞬转表达筛选
- Stable cell line generation/稳定细胞系建立
Recombinant protein expression and purification Services/酵母表达系
○ 重组酵母表达质粒构建/Generation of Recombinant Plasmid
○ 重组质粒电转化/Expression Test in Host
○ 重组表达子筛选/Soluble Expression Test
○ 小规模蛋白表达和纯化（2L培养基培养产物）/Small Scale Expression and Purification (2L Cell Culture)
○ 大规模蛋白表达和纯化（10L以上）/Scale Up Expression and Purification
- Computational Biology & Molecular Modeling/计算生物学和分子模型构建
- Structural-Based Drug Design (SBDD)/基于结构的药物设计
- Target family analysis: using prior knowledge (specificity)/目标族群分析：利用优先知识（特异性）
- Homology modeling/同源建模
- Structural-Based Drug Design (SBDD)/基于结构的药物设计
- Quantitative structure-activity relationship (QSAR)/定量构效关系
- Virtual drug screening/计算机虚拟筛选
- Pharmacophore match for docking-based virtual screen/药物匹配对接的虚拟筛选
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- Hypothesis-driven docking/假设驱动的对接
- Enhancing results of virtual screens using data mining/提高使用数据挖掘的虚拟筛选的结果
- De novo drug design/全新药物设计
详情请查看美迪西官网www.medicilon.com.cn
