使用HUVEC细胞进行血管生成实验，并用LONZA核转仪转染anti-VEGFR2 siRNA

Srinivasan Kokatam1, Kanchan Tiwari1, Jenny Schroeder2, Andrea Toell2, Lubna Hussain3, Preeti Kapoor1.

1. Lonza India Pvt Ltd, Hyderabad, India; 2. Lonza Cologne GmbH, Cologne, Germany; 3. Lonza Walkersville, Inc., Walkersville, MD, U.S.A.

 

 

 

1. 使用pmaxGFP质粒进行进行预实验，优化HUVEC细胞核转条件；

2. 检测核转条件对HUVEC细胞的Tube formation是否有影响；

3. 转染siRNA，确定对细胞活力影响最小的最佳浓度，确定检测蛋白表达降低的最佳时间点；

4. 评价siRNA对HUVEC细胞Tube Formation的影响。

 

图1. HUVEC细胞转染GFP质粒，转染效率约为82%，对细胞形态无明显影响：

图2. HUVEC细胞进行核转后，对Tube formation无明显影响：

图3. Anti-VEGFR2 siRNA转染后的细胞对VEGF无反应，证明siRNA发挥作用。

 

 

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