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直接从生物体获取的组织,一般需要将其消化成单个细胞才能进行体外培养。这种直接从离体组织获得的细胞,更接近于生物体内的生活状态,且生物性状尚未发生很大改变,因此在药物筛选、细胞移植、类器官培养、肿瘤研究等众多领域备受欢迎。但组织消化过程中常遇到多种问题,例如消化不完全、细胞死亡率高等。如何克服这些问题,其实消化酶的选择是关键。 |
来了解组织消化过程中常用的酶吧~ |
1.胰蛋白酶 |
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胶原酶类型及适用范围表 |
3.透明质酸酶 |
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表一:正常组织消化用酶列表 |
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表二:肿瘤组织消化用酶列表 |
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