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蛋白质晶体结构测定：结晶条件筛选，蛋白质共结晶及小分子浸泡，三维结构解析：Se-MAD法和分子置换
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| 服务商： 上海美迪西生物医药有限公司 | 查看该公司所有服务 >> |
蛋白质晶体结构测定
> 结晶条件筛选
> 蛋白质共结晶及小分子浸泡
> 三维结构解析：Se-MAD法和分子置换
美迪西各种结晶体图：
美迪西结晶方法：
1、Multiple constructs into multiple vector
• N- and C- terminal truncation
• Protein mutation of surface residue
• Remove of flexiable regions
2、Expression & Purification of Protein
• Expression & solubility Test
(Co-expression with chapaton or ligand)
• Pilot purification and optimization
(AC, IEX, HIC, SEC ,co-purification with ligand,
buffer optimization)
• Scale-up production (10-50 mg)
• Protein concentration (2-20 mg/ml)
3、QC of target protein
• Mass spectroscopy
• SDS-PAGE
• HPLC (Size exclusion column behavior
4、Crystallization trays set-up
• Around published condition
(5 mg compound)
• Kits screen (10-20 mg)
5、Optimization
• pH and pricipitatant
• Additive screen
• Seeding
• Redesign constructs
6、Diffraction-quality crystal
详情请查看美迪西官网www.medicilon.com.cn
