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当前位置 > 产品目录 > 生理/病理/药理/毒理 > 代谢监测 > 斑马鱼高通量呼吸代谢测量系统
斑马鱼高通量呼吸代谢测量系统
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产地/品牌:中国\易科泰产品类别:代谢监测
型       号:斑马鱼高通量 最后更新:2025-2-22
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      荧光光纤氧气测量技术具有高精确度、高可靠性、响应时间短、适用于气相和液相等优势,因此随着技术的问世,精确、高通量测量微小生物的呼吸和评估其能量代谢成为可能。高通量呼吸测量系统基于荧光光纤氧气测量技术,能够对斑马鱼的胚胎及幼鱼进行测量,测定其耗氧量,进而评估其代谢水平。系统在生物医学、实验生物学、污染生态学与环境毒理学、环境科学、气候变化研究等领域具有越来越重要的应用价值。

       系统由内置荧光光纤氧气传感器的微型呼吸室、氧气测量主机及数据采集分析软件组成,可对96个通道的样品进行同步测量。
 
功能特点
氧气测量高精度、高可靠性、低功耗、低交叉敏感性、快速响应时间
轻松校准
非侵入性和非破坏性测量
紧凑设计,适用于温控培养箱和/或摇床 

技术参数
  • 检测技术:光纤氧传感器技术。
  • 适用场景:原位检测,可在培养箱里或摇床上使用,便于温度控制。
  • 呼吸室:透明聚苯乙烯材质,支持预消毒处理,可重复使用。
  • 氧气测量主机:单个重670 g,162 x 102 x 32 mm
  • 主机内置温度传感器:0-50°C,分辨率0.012°C,精度±0.5°C
  • 主机内置压强传感器:300-1100mbar,分辨率0.11mbar,精度±6mbar
  • 最大采样频率:单通道激活时可达10-20次每秒
8. 氧气测量精度:±0.1% O2@1% O2或±0.05 mg/L@0.44 mg/L
9. 氧气测量分辨率:0.01% O2@1% O2或0.005 mg/L@0.44 mg/L
10. 电源:5VDC,USB供电
11. 响应时间<30s
12. 通道数:96
13. 系统适配其他鱼类的胚胎及幼鱼
14.可选配斑马鱼成鱼的静态及动态呼吸测量系统 

参考文献
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  • Glass, B.H., Jones, K.G., Ye, A.C., Dworetzky, A.G., Barott, K.L., 2023. Acute heat priming promotes short-term climate resilience of early life stages in a model sea anemone. PeerJ 11, e16574.
  • Heuer, R.M., Wang, Y., Pasparakis, C., Zhang, W., Scholey, V., Margulies, D., Grosell, M., 2023. Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 280, 111398.
  • Kämmer, N., Reimann, T., Ovcharova, V., Braunbeck, T., 2023. A novel automated method for the simultaneous detection of breathing frequency and amplitude in zebrafish (Danio rerio) embryos and larvae. Aquatic Toxicology 258, 106493.
  • Louhi, P., Pettinau, L., Härkönen, L.S., Anttila, K., Huusko, A., 2023. Carryover effects of environmental stressors influence the life performance of brown trout. Ecosphere 14, e4361.
  • Mandic, M., Pan, Y.K., Gilmour, K.M., Perry, S.F., 2020. Relationships between the peak hypoxic ventilatory response and critical O2 tension in larval and adult zebrafish ( Danio rerio ). Journal of Experimental Biology jeb.213942.
  • Mathiron, A.G.E., Gallego, G., Silvestre, F., 2023. Early-life exposure to permethrin affects phenotypic traits in both larval and adult mangrove rivulus Kryptolebias marmoratus. Aquatic Toxicology 259, 106543.
  • Moore, B., Jolly, J., Izumiyama, M., Kawai, E., Ryu, T., Ravasi, T., 2023. Clownfish larvae exhibit faster growth, higher metabolic rates and altered gene expression under future ocean warming. Science of The Total Environment 873, 162296.
  • Park, K.-H., Ye, Z., Zhang, J., Hammad, S.M., Townsend, D.M., Rockey, D.C., Kim, S.-H., 2019. 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish. Sci Rep 9, 1138.
  • Ricarte, M., Prats, E., Montemurro, N., Bedrossiantz, J., Bellot, M., Gómez-Canela, C., Raldúa, D., 2023. Environmental concentrations of tire rubber-derived 6PPD-quinone alter CNS function in zebrafish larvae. Science of The Total Environment 896, 165240.
  • Saputra, F., Lai, Y.-H., Roldan, M.J.M., Alos, H.C., Aventurado, C.A., Vasquez, R.D., Hsiao, C.-D., 2023. The Effect of the Pyrethroid Pesticide Fenpropathrin on the Cardiac Performance of Zebrafish and the Potential Mechanism of Toxicity. Biology 12, 1214.
  • Schuster, L., Cameron, H., White, C.R., Marshall, D.J., 2021. Metabolism drives demography in an experimental field test. Proceedings of the National Academy of Sciences 118, e2104942118.
  • Scovil, A.M., Boloori, T., de Jourdan, B.P., Speers-Roesch, B., 2023. The effect of chemical dispersion and temperature on the metabolic and cardiac responses to physically dispersed crude oil exposure in larval American lobster (Homarus americanus). Marine Pollution Bulletin 191, 114976.
  • Varshney, S., Gora, A.H., Kiron, V., Siriyappagouder, P., Dahle, D., Kögel, T., Ørnsrud, R., Olsvik, P.A., 2023. Polystyrene nanoplastics enhance the toxicological effects of DDE in zebrafish (Danio rerio) larvae. Science of The Total Environment 859, 160457.
  • Varshney, S., Gora, A.H., Siriyappagouder, P., Kiron, V., Olsvik, P.A., 2022. Toxicological effects of 6PPD and 6PPD quinone in zebrafish larvae. Journal of Hazardous Materials 424, 127623.
  • Varshney, S., Lundås, M., Siriyappagouder, P., Kristensen, T., Olsvik, P.A., 2024. Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model. Ecotoxicology and Environmental Safety 269, 115796.
  • Wang, Y., Pasparakis, C., Grosell, M., 2021. Role of the cardiovascular system in ammonia excretion in early life stages of zebrafish ( Danio rerio ). American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 321, R377–R384. 
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