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| 销售商： 上海雅吉生物科技有限公司 | 查看该公司所有产品 >> |
英文名称HSP90 beta
中文名称热休克蛋白90β/HSP90 β 单克隆抗体
别 名HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Heat shock protein HSP 90-beta; Heat shock 84 kDa.
研究领域肿瘤 免疫学 信号转导 转录调节因子
抗体来源Mouse
克隆类型Monoclonal
克 隆 号2A2
交叉反应Human, Mouse, Rat,
产品应用WB=1:500-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量84kDa
细胞定位细胞浆
性 状Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HSP90 beta:
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Subunit:
Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications:
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
Similarity:
Belongs to the heat shock protein 90 family.
SWISS:
P08238
Gene ID:
3326
Database links:
Entrez Gene: 3326 Human
Entrez Gene: 15516 Mouse
Entrez Gene: 301252 Rat
Omim: 140572 Human
SwissProt: P08238 Human
SwissProt: P11499 Mouse
SwissProt: P34058 Rat
Unigene: 509736 Human
Unigene: 2180 Mouse
Unigene: 98667 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
HSP是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关，HSP90β能够抑制细胞凋亡与分化。
| 产品图片 |
 Sample:
Hela KO HSP90 beta(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample:
K562 (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample:
Hela (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: U2os (Human) Cell Lysate at 30 ug Lane 7: A549 (Human) Cell Lysate at 30 ug Lane 8: HL60 (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: Testis (Mouse) Lysate at 40 ug Lane 11: Testis (Rat) Lysate at 40 ug Lane 12: Cerebrum (Mouse) Lysate at 40 ug Lane 13: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 86 kD  Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
 Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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试剂盒售后：本公司出售的试剂盒均保质保量，质量问题均可免费退换，另提供免费代测服务。
细胞售后：
1. 细胞运输丢失、瓶身破损、培养液严重漏液等，重发；
3. 细胞收到当天以及第2,3天请拍照，未告知的视为产品合格。4-10天内出现问题，请提供细胞照片和细胞出现问题的照片以及细胞相关操作的详细步骤，并跟我公司人员及时沟通判定是否重发，具体可以参照我官网或者随货说明书相关售后条款。
细胞售后：
1. 细胞运输丢失、瓶身破损、培养液严重漏液等，重发；
3. 细胞收到当天以及第2,3天请拍照，未告知的视为产品合格。4-10天内出现问题，请提供细胞照片和细胞出现问题的照片以及细胞相关操作的详细步骤，并跟我公司人员及时沟通判定是否重发，具体可以参照我官网或者随货说明书相关售后条款。
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