S100B蛋白单克隆抗体

中文名称S100B蛋白单克隆抗体

别    名NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta (neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta; S100 β; S100-β; S100β.  

研究领域肿瘤  细胞生物  神经生物学  细胞周期蛋白  细胞分化  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号1C6

交叉反应Human, Mouse, Rat, 

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量10kDa

细胞定位细胞核 细胞浆 

性    状Liquid

浓    度1mg/ml

免 疫 原Recombinant human S100B protein: 2-92/92aa (N-6x His-Tag) 

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

 

Function:

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

 

Subunit:

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1.

 

Subcellular Location:

Cytoplasm. Nucleus.

 

Tissue Specificity:

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

 

Similarity:

Belongs to the S-101 family.

Contains 2 EF-hand domains.

 

SWISS:

P04271

 

Gene ID:

6285

 

Database links:

Entrez Gene: 6285 Human

 

Omim: 176990 Human

 

SwissProt: P04271 Human

 

Unigene: 422181 Human

 

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

结构蛋白（Structural Proteins）

S100B是一组可溶性、相对低分子质量、高酸性蛋白，属神经胶质细胞特异性蛋白，亦可存在于神经纤维中。在研究肠神经系统的形态特征及结构特征中，S100B有良好的表达功能。 S100B蛋白其生理作用尚不清楚，可能与细胞内钙离子媒介的调节作用有关。S100B蛋白可存在于神经鞘瘤，成软骨细胞瘤、软骨肉瘤、骨肉瘤、神经胶质瘤、郎格罕斯细胞瘤、一些癌肿和黑素细胞肿瘤等。

在免疫组化中，除了恶性黑素瘤皮损，S100B蛋白还在良性黑素细胞痣中增加，如：蓝痣、普通后天痣、雀斑样痣、Spitz痣、气球状细胞痣、色素性梭形细胞痣和发育不良痣中表达。还有报道S100B蛋白的染色强度与肿瘤细胞的黑素含量呈负相关。结缔组织增生性的或亲神经的黑素瘤不易染色。

产品图片

Sample: Lane 1: Cerebral cortex (Rat) Lysate at 40 ug Lane 2: Spinal cord (Mouse) Lysate at 40 ug Lane 3: Spinal cord (Rat) Lysate at 40 ug Lane 4: Cerebellum (Mouse) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-S100B (bsm-10832M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 11 kD Observed band size: 9 kD Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human neurofibroma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M 1C6) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M 1C6) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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