神经元核抗原重组兔单克隆抗体

别    名 FOX3; NeuN; neuronal nuclei antigen; neuronal nuclei; Vertebrate neuron-specific nuclear protein; Neuna60; Neuronal nuclear antigen A60; RNA binding protein fox-1 homolog 3 isoform 1; RNA binding protein, fox 1 homolog (C. elegans) 3; Rbfox3; D11Bwg0517e; Fox-3; Fox3; Hrnbp3; RP23-159O6.5; novel protein; Fox-1 homolog C; fox1 homolog C; FLJ56884; FLJ58356; hexaribonucleotide binding protein 3 RFOX3_HUMAN.   

研究领域 细胞生物  神经生物学  转运蛋白  表观遗传学   

抗体来源 Rabbit 

克隆类型 Monoclonal 

克 隆 号 12C3 

交叉反应 Mouse, Rat,  (predicted: Human, ) 

产品应用 WB=1:1000-2000 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 34kDa 

细胞定位 细胞核 细胞浆  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human NeuN:  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.

 

Function:

RNA-binding protein t

产品图片

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Cerebrum (Rat) Lysate at 40 ug Lane 4: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-NeuN (bsm-52268R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/50 kD Observed band size: 46/50 kD

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