CHI Scientific研发的成纤维细胞生长抑制体系—FibrOut™，包含多种生化成分和有机试剂，能够抑制成纤维细胞在原代细胞培养中的成长，进而促进靶细胞生长。每一种成纤维细胞抑制剂是组织和细胞特异性的，适用于相应的PrimaCellTM原代细胞培养体系，可以为您提供理想状态的原代细胞。
规格型号
产品体系
人成纤维抑制剂
小鼠成纤维抑制剂
大鼠成纤维抑制剂
禽类成纤维抑制剂
质量检测
每1ml FibrOut™可以混合于500ml培养基，每批试剂均经过成纤维细胞抑制性能测试。
论文引用
Companion-Probe & Race platform for interrogating nuclear protein and migration of living cells
Hong Sun 1, Zaizai Dong 2, Qingyang Zhang 3, Bing Liu 4, Shi Yan 4, Yusen Wang 1, Dedong Yin 5, Peigen Ren 6, Nan Wu 4, Lingqian Chang 7
Biosens Bioelectron. 2022 Aug 15;210:114281. DOI: 10.1016/j.bios.2022.114281
.....with 15% FBS and FibrOutTM fibroblast inhibitor (4-21564, CHI Scientific) (Hang et al., 2022). The above cells were all cultured in a cell incubator (CCL-170JB-8, ESCO) with the condition of 37 ◦C and 5% CO2.
更多
1) [HTML] Cancer/testis antigens expression during cultivation of melanoma and soft tissue sarcoma cells
A Danilova, V Misyurin, A Novik, D Girdyuk… - Clinical Sarcoma …, 2020 - Springer
… When cells reached sub-confluence, they were dispersed with 0.25% trypsin–0.02% EDTA
(Sigma-Aldrich, USA) and seeded in a new culture plate. Fibroblast growth-inhibitory cocktail
Human FibrOut™ 9 (Chi Scientific Inc., USA) was used for fibroblast growth prevention …
2) Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization
HJ Chen, ML Shuler - Cancer Driver Genes, 2019 - Springer
… 5. Media with fibroblast inhibitory reagent: Colonic epithelial cell growth medium with 1%
fibroblast inhibitory reagent (Human Colon FibrOut™ from CHI Scientific) for the first two to three
passages of primary cultures to prevent the outgrowth of fibroblasts. 6 …
3) A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells
HJ Chen, P Miller, ML Shuler - Lab on a Chip, 2018 - pubs.rsc.org
… ng ml −1 R-spondin 1 (R&D systems) and 50 ng ml −1 Noggin (Peprotech, NJ) in collagen-I coated
flasks (BD scientific, NJ) and incubated in a humidified incubator at 5% CO 2 and 37 °C. After
48 hour culture, fibroblast inhibitory reagent (Human Colon FibrOut™, CHI Scientific …
4) [PDF] A novel way of harvesting a large population of endothelial cells for clinical and experimental use
AO Ilesanmi - Biomed Res Rev, 2018 - pdfs.semanticscholar.org
… If it is eventually decided that fibroblasts should be eliminated completely, there is a commercial
product, the Human Vascular Endothelial PrimaCell™ system that includes an effective fibroblast
elimination system, Vascular Endothelial FibrOut™ (www.chiscientific.com) …
5) [PDF] gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene, 36 (1), pp. 110-121.(doi: 10.1038/onc. 2016.181) This is the author's final accepted version …
F Iltzsche - core.ac.uk
… ml fresh media. Tissue was broken up by pipetting and the cell suspension was cultured in media
supplemented with Mouse FibrOut™ 9 (CHI Scientific). Cells were cultured until confluency was
reached, genotyped and stored for further processing. Antibodies …
6) Very long chain fatty acid β-oxidation in astrocytes: contribution of the ABCD1-dependent and-independent pathways
M Morita, S Shinbo, A Asahi… - Biological and …, 2012 - jstage.jst.go.jp
… The MessageMuter shRNAi Production kit was from EPICENTRE. FibrOut (System 4,
Mouse) was purchased from CHI Scientific (Maynard, MA, USA). Antimycin A and ro-
tenone were purchased from Sigma-Aldrich (St. Louis, MO, USA) …
7) Requirement of the NF-κB subunit p65/RelA for K-Ras–induced lung tumorigenesis
DS Bassères, A Ebbs, E Levantini, AS Baldwin - Cancer research, 2010 - AACR
8) p38α MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation
JJ Ventura, S Tenbaum, E Perdiguero, M Huth… - Nature …, 2007 - nature.com
bio-equip.com
齐氏生物科技有限公司成立于2009年，是一家专注于原代细胞培养和筛选、体外药物代谢和药物间相互作用评价产品的公司，为全球药物公司、药物研究所、疾控中心、新药安评中心和各类科研机构提供符合标准规范产品，致力于打造原代细胞生物制药研发和筛选平台。
公司对细胞的培养提供全程服务，产品涵盖原代细胞、细胞培养试剂、体外代谢试剂等。同时公司还提供药物研发以及原代细胞药物筛选等多项生物医药外包服务。每个科研团队都配备了多名经验丰富的专家，可以为您的科学研究提供高效优质的服务。公司凭借在原代细胞领域强大的技术实力和丰富的经验，正着力创建新一代以原代细胞为基础的生物制药筛选平台，用于甄别和筛选候选药物，检测药物毒性，从而节省新药的开发时间和开发成本，同时提高新药的治愈率。
细胞生物学产品专家——齐氏生物科技，您值得信任的合作伙伴！
