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气体孵育系统
英文名称:总访问:1309
国产/进口:进口半年访问:13
产地/品牌:bidi产品类别:细胞分析仪
型       号:11920 最后更新:2019-4-3
货       号:
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Applications:

1.Live cell imaging that requires incubator conditions on the microscope, for example:
Chemotaxis
Angiogenesis
Cell migration, proliferation, wound healing
Cell culture under flow

2.Imaging applications with extra focus stability, for example: 
TIRF  
Confocal microscopy 

3.Anaerobic conditions or oxidative stress


Technical Features:

1.Precise and reliable gas incubation for O2 and CO2

2.Active and fast humidification - no evaporation

3.Uses pressurized air to create the gas flow – no vibrations

4.Optional air pressure generator available (for when pressurized air is not obtainable)

 图3.jpg

The ibidi Gas Incubation System provides both humid and CO2-rich air for stage-top incubators like the ibidi Heating System. The gas mixture is continuously flushed through the stage top incubator, ensuring a maximum humidity and an optimal pH for CO2-buffered liquids.

图4.jpg

Spheroid of breast cancer cells with living cells (green) and apoptotic center (red) due to oxygen depletion.

 

Components of the ibidi Gas Incubation System

图5.jpg

 

ibidi Gas Mixer for CO2 and O2

1.Precise and reliable gas incubation

2.Active and fast humidification without evaporation (patent pending technology)

3.Suitable for various experimental conditions (e.g., pH or hypoxia)

4.Fully compatible with the ibidi Heating Systems

5.Uses pressurized air to create the gas flow – no vibrations

6.Optional air pressure generator available (for when pressurized air is not obtainable)

图6.jpg

Humidifying Column

1.Part of the ibidi Gas Incubation System

2.Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation

3.Active humidity control inside the attached ibidi Heating System

图7.jpg

 

The ibidi Gas Incubation System upgrades your ibidi Heating System to a complete stage top incubator.

Location/Tissue Oxygen Partial Pressure p(O2) Corresponding O2 in 
Cell Culture Gas Mix (%)
Inspired air 21 kPa (=160 mm Hg) 21%
Alveolus 14 kPa (=104 mm Hg) 14%
Arterial blood 14 kPa (=104 mm Hg) 14%
Veneous blood 5 kPa (=40 mm Hg) 5%
Interstitial fluid 5 kPa (=40 mm Hg) 5%
Healthy tissue 2-3 kPa (=15- 23 mm Hg) 2-3%
Tumor without blood vessels  
   Outside 1 kPa (=7.5 mm Hg) 1%
   Center 0 kPa (=0 mm Hg) 0%
Normoxia conditions 2 - 11 kPa (=15 - 82 mm Hg) 2-11%
Hypoxia conditions 0 - 1 kPa (=0 - 7.5 mm Hg) 0-1%
Cell culture incubator (5% CO2) 20 kPa (=150 mm Hg) 20%

图8.jpg

        图9.jpg 

                     图10.jpg

图11.jpg

ibidi Gas Incubation System

Compatible with:

1.ibidi Heating System, Universal Fit

2.ibidi Heating System, Multi-Well Plates

 

Overview of the ibidi Heating & Incubation System, CO2 and O2 Control

Gas Supply (Input)

Specifications:

Gas Supply (Input)
Pressurized Air 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
CO2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
N2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)

 

Tubing for all 
gasses (Supplied with the system)

PUR/PUN tubing
4 mm inner diameter
6 mm outer diameter
1 mm wall thickness

ibidi Gas Mixer

Specifications:

CO2 Control  
CO2 Control Range 0.1% - 20%
Accuracy 0.1% - 0.5% (absolute)
   
O2 Control  
O2 Control Range 0% - 21%
Accuracy 0.5 % (absolute)
   
Gas Flow  
Control Range 5 - 20 l/h
 

Humidifying Column

Specifications:

Humidity Control
Control Range 20% - 99% (Rel. Humidity)
Accuracy 1% (absolute)
Water Refill Every 7 days
 

 

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