急性早幼粒白血病标志物检测试剂盒

急性早幼粒白血病分子标志物检测试剂盒

Leukemia(Promyelocytic, Acute) Molecular Marker (PRKAR1A and AKT1) Detection Kit 
 
Description: The kit provides a means of performing Leukemia (Promyelocytic, Acute) detection method based on kinase activity assays. Three targets of molecular marker (PRKAR1A and AKT1) are involved in Leukemia (Promyelocytic, Acute). This kit includes Streptavidin Coated Plates and specific Substrate-Peptides (biotinylated peptide substrates) for detection of the activity of PRKAR1A and AKT1 which indicate the prognosis of Leukemia (Promyelocytic, Acute). 


Molecular Markers in Leukemia (Promyelocytic, Acute)

 

Disease	Molecular Marker	Gene ID	Gene Disease Relation
Leukemia (Promyelocytic, Acute)	PRKAR1A	5573	marker/mechanism
Leukemia (Promyelocytic, Acute)	AKT1	207	marker/mechanism
Leukemia (Promyelocytic, Acute)	AKT1	207	marker/mechanism

- Molecular markers for drug discovery and life science research (mechanism).

Peptide Core Sequence: 

Specific Substrate-Peptides for Leukemia (Promyelocytic, Acute) Molecular Marker

 

Molecular Marker	Substrate-Peptide	Residue	Reaction Type
PRKAR1A	Peptides Mix	Ser-336	Phosphorylation
AKT1	Peptides Mix	Ser-429	Phosphorylation
AKT1	Peptides Mix	Ser-196	Phosphorylation

Label of Substrate-Peptides:  Biotinylated

Quality Control: The substrate peptide was selected using our Leukemia (Promyelocytic, Acute) Detection Kit. The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Storage: 
Store at 4 °C, Avoid Freeze.


Background:

cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. Three alternatively spliced transcript variants encoding the same protein have been observed. RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. Multiple alternatively spliced transcript variants have been found for this gene. AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
 
联系方式：
业务手机：18221844720   电话：021-34315602  邮箱：biopharmsoft@sina.com

 

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