PIK3CA(Q546K) Mouse Monoclonal Antibody

 

PIK3CA(Q546K) [CR-M26138] [100 µL] Catalog Number:  CR-M26138 Gene Symbol:  PIK3CA Description:   Anti PIK3CA(Q546K) Mouse Monoclonal Antibody Background:   Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform. Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Belongs to the PI3/PI4-kinase family. Located to cytosol, lamellipodium and phosphatidylinositol 3-kinase complex. Immunogen:   A synthetic peptide from the internal region of PIK3CA(Q546K) of human origin. Applications:   ELISA, WB, IHC Recommended Dilutions:    ELISA: 1:1000-1:5000  WB: 1:100-1:1000  IHC: 1:50-1:100 Concentration:   0.2 mg/ml Host:   Mouse Format:   Liquid Clonality:   Monoclonal Isotype:    Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  recognizes Q546K mutant, but not wildtype PIK3CA of vertebrates. Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

 Western blot analysis of PIK3CA wild type and Q546K proteins. Equal amount of purified PIK3CA(Q546K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti- PIK3CA(Q546K) mouse monoclonal antibody(Cat. # CR-M26138).

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