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销售商： 上海伯建生物科技有限公司 | 查看该公司所有产品 >> |
Wmicrotracker® SMART is our new technology that allows you to obtain real-time information on the behavior of small animal populations over time and space. The "Equipment + Software" system is optimized to quantify the speed and distance traveled of small organisms in 35 mm Petri dishes. |
Many |
Many biological assays are complex to perform, requiring great manipulation skills and analysis time. This makes the work tedious and limits experiments based on their level of difficulty. Therefore, at Phylumtech, our vision is to provide unique, agile, and real-time solutions that enable immediate, reliable, and reproducible results. Founded in 2009 as a joint venture between the public and private sectors, we combine the latest advances in science and research with cutting-edge technologies
After years of experience, we are committed to the development of new products that are more accessible and have WIFI connectivity. The Wmicrotracker® SMART, based on non-invasive technology of infrared microbeams and camera detection, developed with CONICET and protected by international patents. SMART allows the quantification of activity, trajectory, and speed of small organisms in a 35 mm diameter Petri dish format. The detection system is compatible with small organisms such as C.elegans and other nematodes.
HOW IT WORKS? The system has the ability to work in 2 different modes: 1) Infrared Images, with the plate upside down: This arrangement allows the tracking of the route of multiple C.elegans (or similar-sized worms) using NGM-type solid medium cultures. The method is based on an optical phenomenon of Silhouette Amplification by Infrared Refraction, where infrared light waves refract at the worm-agar interface, generating an amplified image that will be captured by a sensitive HD optics/camera system. The digital image processing will be carried out by software specially designed for real-time data acquisition. 2) Grid of infrared microbeams, with the plate upside up: It allows the quantification of the behavior of multiple organisms of size > 0.1 mm using solid, liquid or air medium cultures. It also allows defining the activity area on the plate for use in chemotaxis experiments. This patented method is based on detecting movement through the light scattering caused by a grid of infrared microbeams SOFTWARE 3.Calculation of parameters 2. Tracking of organisms
1. Recognition of organisms |
Infrared Amplification INFRARED LIGHT CAMERA WIFI OPTIC WITH FOCUS ADJUSTMENT DIFUSSER |
The calculated distances correlate very well with manual processing
In the current version, the recognition of worms is 80%
APLICATIONS Neurodegeneration-Lifespan-Toxicity-Microbiota-RNAi-Hypoxia |
LIFESPAN The graph shows the tracks of individual worms on the plate and their decrease with age. 20 adult C.elegans were cultured at 25°C on a 35 mm Petri dish with NGM+FuDR 80 μM. Data acquisition was performed once a day, using a 5-minute acquisition interval. The plates were subjected to mechanical stimulation of "tap" before each measurement. It can be seen that the 50% velocity rate decreases earlier compared to when worms become immobile. |
TOXICITY Example of kinetics for levamisole treatment. 25 worms were cultured in 35mm Petri dishes containing NGM and increasing concentrations of levamisole [0 to 200 uM]. The plates were recorded for 5 minutes every hour, after mechanical stimulation by "tap". The graphs show the decrease in worm movement speed. |
Components Included |
Measurements Requirements |
