NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

NEXTflex™ 16S V4 Amplicon-Seq Library Prep Kit 2.0 专为微生物16S核糖体RNA（rRNA）基因 V4高变区文库制备设计的一款试剂盒，支持多重扩增子测序建库。兼容于Illumina Miseq测序平台双端测序，是监测微生物群落种群波动的理想实验方案。

Figure 1. 使用NEXTflex™ 16S V4 Amplicon-Seq Library Prep Kit 2.0 建库，对16S V4 reads分析测量，样本为犬类粪便中提取的20ng DNA。

 

低PCR偏差，高目标序列读取率实验流程

NEXTflex 16S V4 Amplicon-Seq Kit 2.0 实验流程中采用2步PCR，以增加样本特异性index，减少扩增子测序中的目标序列脱靶率。

 

低样本起始量

NEXTflex 16S V4 Amplicon-Seq Library Prep Kit 2.0进行建库时所需样本量为 1 ng–50ng基因组DNA。

 

产品特点：

• 快速-2小时文库制备
• 简单-两步完成整个流程
• 低样本起始量-只需1ng的样品
• 低PCR偏差，高目标序列读取率
• 测序引物都包含在此试剂盒中
• 多达384种接头用于多重测序
• 匹配Sciclone NGS 和NGSx自动工作站
• 经Illumina测序平台验证

 

产品列表：

货号	产品名称	规格
NOVA-4203-02	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0 (12 Barcodes)	24 RXNS
NOVA-4203-03	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0 (48 Barcodes)	96 RXNS
NOVA-4203-04	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0(Barcodes 1-96)	192 RXNS
NOVA-4203-05	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0(Barcodes 97-192)	192 RXNS
NOVA-4203-06	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0(Barcodes 193-288)	192 RXNS
NOVA-4203-07	NEXTflex™ 16S V4 Amplicon-Seq Kit 2.0(Barcodes 289-384)	192 RXNS

 

KIT CONTENTS

• NEXTFLEX® PCR Master Mix
• NEXTFLEX® 16S V4 PCR I Primer Mix
• NEXTFLEX® PCR II Barcoded Primer Mix
• Resuspension Buffer
• Nuclease-free Water

REQUIRED  MATERIALS NOT PROVIDED

• 1 ng – 50 ng high-quality genomic DNA in up to 36 µL nuclease-free water for each library
• 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
• Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
• Agencourt® AMPure® XP 5 mL (Beckman Coulter® Genomics, Cat # A63880)
• Magnetic Stand – 96 (Thermo Fisher® Scientific, Cat # AM10027) or similar
• Thermocycler
• 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
• Nuclease-free barrier pipette tips
• Vortex
• 80% Ethanol, freshly prepared (room temperature)

 

NEXTflex 16S V4 Amplicon-Seq Kit 部分引用文献

• Bekker, V., Zwittink, R. D., Knetsch, C. W., Sanders, I. M., Berghuis, D., Heidt, P. J., . . . Kuijper, E. J. (2019). Dynamics of the Gut Microbiota in Children Receiving Selective or Total Gut Decontamination Treatment During Hematopoietic Stem Cell Transplantation. Biology of Blood and Marrow Transplantation. doi:10.1016/j.bbmt.2019.01.037.
• Bruce-Keller, A. J., Fernandez-Kim, S., Townsend, R. L., Kruger, C., Carmouche, R., Newman, S., . . . Berthoud, H. (2017). Maternal obese-type gut microbiota differentially impact cognition, anxiety and compulsive behavior in male and female offspring in mice. Plos One, 12(4). doi:10.1371/journal.pone.0175577.
• Davey, M. P., Norman, L., Sterk, P., Huete-Ortega, M., Bunbury, F., Loh, B. K., . . . Smith, A. G. (2019). Snow algae communities in Antarctica – metabolic and taxonomic composition. New Phytologist. doi:10.1111/nph.15701.
• Ford, S. L., Lohmann, P., Preidis, G. A., Gordon, P. S., Odonnell, A., Hagan, J., . . . Hair, A. B. (2019). Improved feeding tolerance and growth are linked to increased gut microbial community diversity in very-low-birth-weight infants fed mothers own milk compared with donor breast milk. The American Journal of Clinical Nutrition,109(4), 1088-1097. doi:10.1093/ajcn/nqz006.
• Luk, B., Veeraragavan, S., Engevik, M., Balderas, M., Major, A., Runge, J., . . . Versalovic, J. (2018). Postnatal colonization with human “infant-type” Bifidobacterium species alters behavior of adult gnotobiotic mice. Plos One, 13(5). doi:10.1371/journal.pone.0196510.
• Luna, R. A., Oezguen, N., Balderas, M., Venkatachalam, A., Runge, J. K., Versalovic, J., . . . Williams, K. C. (2017). Distinct Microbiome-Neuroimmune Signatures Correlate With Functional Abdominal Pain in Children With Autism Spectrum Disorder. Cellular and Molecular Gastroenterology and Hepatology, 3(2), 218-230. doi:10.1016/j.jcmgh.2016.11.008
• Moon, C., Stupp, G. S., Su, A. I., & Wolan, D. W. (2017). Metaproteomics of colonic microbiota unveils discrete protein functions among colitic mice and control groups. doi:10.1101/219782.
• Stalenhoef, J. E., et al. (2017) Fecal Microbiota Transfer for Multidrug-Resistant Gram-Negatives: A Clinical Success Combined With Microbiological Failure. Open Forum Infectious Diseases, 4(2). doi:10.1093/ofid/ofx047.
• Svensson, K., Paruch, L., Gaby, J. C., & Linjordet, R. (2018). Feeding frequency influences process performance and microbial community composition in anaerobic digesters treating steam exploded food waste. Bioresource Technology, 269, 276-284. doi:10.1016/j.biortech.2018.08.096.
• Sylvia, K. E., Deyoe, J. E., & Demas, G. E. (2018). Early-life sickness may predispose Siberian hamsters to behavioral changes following alterations of the gut microbiome in adulthood. Brain, Behavior, and Immunity, 73, 571-583. doi:10.1016/j.bbi.2018.07.001.
• Whon, T. W., Chung, W., Lim, M. Y., Song, E., Kim, P. S., Hyun, D., . . . Nam, Y. (2018). The effects of sequencing platforms on phylogenetic resolution in 16 S rRNA gene profiling of human feces. Scientific Data, 5, 180068. doi:10.1038/sdata.2018.68.
• Wotzkas, S. Y., Kreuzer, M., Maier, L., Zuend, M., Schlumberger, M., Nguyen, B., . . . Misselwitz, B. (2018). Microbiota stability in healthy individuals after single-dose lactulose challenge – a randomized controlled study. doi:10.1101/424531.
• Zhao, R., Yang, W., Pei, F., Zhao, L., & Hu, Q. (2018). In vitro fermentation of six kinds of edible mushrooms and its effects on fecal microbiota composition. Lwt, 96, 627-635. doi:10.1016/j.lwt.2018.06.012.

技术服务：
1、DAP-seq：高通量检测转录因子或DNA结合蛋白在基因组上的结合位点。
2、酵母单杂交：研究蛋白质和特定DNA序列相互作用。
3、酵母双杂交：检测已知蛋白质之间的相互作用，鉴定与已知蛋白相互作用的未知蛋白。
4、EMSA：鉴定或验证DNA结合蛋白和DNA序列的相互作用。
5、ChIP-seq：高效检测重组蛋白、转录因子在基因组的结合位点。
6、DAP-seq与RNA-seq联合分析： 分析转录因子的靶基因在RNA-seq数据中的表达变化，深入挖掘DAP-seq和RNA-seq测序数据，增加转录组测序的分析深度。
7、DNA-pull down：鉴定与DNA结合的蛋白。
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