NEXTFLEX® DNA Barcodes

Prevent Poor Reads Caused by Single Base Errors

The NEXTFLEX® DNA barcodes for Ion Torrent™ sequencing platforms can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® DNA barcodes use an indexed adapter with a 10 nt unique sequence, allowing for proper differentiation between samples and preventing poor reads caused by single base errors introduced during PCR. Up to 64 samples can be multiplexed using these adapters. The NEXTFLEX® DNA barcodes undergo stringent quality controls and are functionally validated by sequencing.

 

These barcodes are compatible with the NEXTFLEX® DNA library prep kits for Ion Torrent™ sequencers, the NEXTFLEX® Cell Free DNA-seq kit for Ion Torrent™ sequencers, and other genomic and ChIP-seq DNA library prep protocols compatible with the Ion Torrent™ PGM™, Ion Proton™, Ion S5™, and Ion S5™ XL sequencers.

 

产品特点：

• Up to 64 samples can be multiplexed
• Compatible with DNA-Seq and ChIP-Seq library prep on the Thermo Fisher® Scientific Ion S5™, Ion S5™ XL, Ion PGM™, and Ion Proton™ sequencing platforms
• Considerably reduce your per-sample sequencing cost using barcoded multiplexing
• Increase your sequencing scale by pooling 100s of samples on a single flow cell

 

产品列表：

货号	产品名称	规格
NOVA-401001	NEXTFLEX® DNA Barcodes for Ion Torrent™ Sequencers-8	48 RXNS
NOVA-401002	NEXTFLEX® DNA Barcodes for Ion Torrent™ Sequencers-16	96 RXNS
NOVA-401003	NEXTFLEX® DNA Barcodes for Ion Torrent™ Sequencers-32	192 RXNS
NOVA-401004	NEXTFLEX® DNA Barcodes for Ion Torrent™ Sequencers-64	384 RXNS

 

试剂盒组分：

• NEXTFLEX® DNA P1 Adapter (6.25 µM)
• NEXTFLEX® DNA Barcode Adapters (6.25 µM)
• NEXTFLEX® Primer Mix (12.5 µM)

参考文献：

• Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.
• Argueta, W. C. (2015) Comparison of Next Generation Sequencing Methodology on the Ion PGM System Performance versus that on the Sanger Sequencing Method for HV1 and HV2 Regions of mtDNA. University of North Texas Health Science Center. UNTHSC Scholarly Repository.
• Lundberg, P. et al. (2014) Clonal evolution and clinical correlates of somatic mutations in myeloproliferative neoplasms. Blood 123(14): 2220-2228.
• Raphael, B. H., Shirey, T. B., Lúquez, C. and Maslanka, S. E. (2014) Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains. BMC Microbiol. 192: 14.

技术服务：
1、DAP-seq：高通量检测转录因子或DNA结合蛋白在基因组上的结合位点。
2、酵母单杂交：研究蛋白质和特定DNA序列相互作用。
3、酵母双杂交：检测已知蛋白质之间的相互作用，鉴定与已知蛋白相互作用的未知蛋白。
4、EMSA：鉴定或验证DNA结合蛋白和DNA序列的相互作用。
5、ChIP-seq：高效检测重组蛋白、转录因子在基因组的结合位点。
6、DAP-seq与RNA-seq联合分析： 分析转录因子的靶基因在RNA-seq数据中的表达变化，深入挖掘DAP-seq和RNA-seq测序数据，增加转录组测序的分析深度。
7、DNA-pull down：鉴定与DNA结合的蛋白。
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