NEXTFLEX® ChIP-Seq Barcodes

Increase Your Sequencing Scale

The NEXTFLEX® ChIP-Seq Barcodes are adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes are available in sets of 6, 12, 24, and 48 unique adapters. The ability to pool samples in an efficient way significantly decreases hands-on time while providing robust data quality. These barcodes can be used with single, paired-end, and multiplex reads. These adapters are compatible with the NEXTFLEX® ChIP-Seq and the NEXTFLEX® Rapid DNA-Seq 2.0 workflows.

 

Avoiding Registration Failure with Low Level Multiplexing

Registration failure could occur if the color balance was not maintained between the red and green lasers (used to sequence A/C bases and G/T bases, respectively). Read our recent blog post, Tech Tips – Barcode Recommendations for Low Level Multiplexing, to learn how to avoid registration failure on an Illumina® sequencer caused by lack sufficient index sequence diversity.

 

产品特点：

• 6 nt index contained within adapter sequence
• Up to 48 multiplexed samples (up to 384 reactions per kit)
• Considerably reduce your per-sample sequencing cost by barcoded multiplexing
• Increase your sequencing scale by pooling 100s of samples on a single flow cell
• Compatible with Illumina® next-generation sequencing platforms

 

产品列表：

货号	产品名称	规格
NOVA-514120	NEXTFLEX® ChIP-Seq Barcodes-6	48 RXNS
NOVA-514121	NEXTFLEX® ChIP-Seq Barcodes-12	96 RXNS
NOVA-514122	NEXTFLEX® ChIP-Seq Barcodes-24	192 RXNS
NOVA-514123	NEXTFLEX® ChIP-Seq Barcodes-48	384 RXNS

试剂盒组分：

• NEXTFLEX® ChIP Adapter (0.6 µM)
• NEXTFLEX® Primer Mix (12.5 µM)

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技术服务：
1、DAP-seq：高通量检测转录因子或DNA结合蛋白在基因组上的结合位点。
2、酵母单杂交：研究蛋白质和特定DNA序列相互作用。
3、酵母双杂交：检测已知蛋白质之间的相互作用，鉴定与已知蛋白相互作用的未知蛋白。
4、EMSA：鉴定或验证DNA结合蛋白和DNA序列的相互作用。
5、ChIP-seq：高效检测重组蛋白、转录因子在基因组的结合位点。
6、DAP-seq与RNA-seq联合分析： 分析转录因子的靶基因在RNA-seq数据中的表达变化，深入挖掘DAP-seq和RNA-seq测序数据，增加转录组测序的分析深度。
7、DNA-pull down：鉴定与DNA结合的蛋白。
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