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植物通用型 riboPOOLs核糖体RNA去除
可去除细胞质(28S, 18S, 5.8S & 5S) 、质体和线粒体 rRNA
广泛覆盖开花植物
适合叶片、种子和根等各种组织部位
植物通用型 riboPOOL 可以覆盖开花植物的主要门类:
包括30 多个目(见下图)常见物种,如下:水稻 · 麻疯树 · 大豆·土豆 · 棉花 · 咖啡·胡萝卜 · 洋蓟 · 橄榄·拟南芥 · 玉米 · 翠菊·小麦 · 苹果 · 橙子·荞麦 · 及其他。
植物通用型 riboPOOL的β测试结果
开花植物的去除效率大于91%。值得注意的是,在原始藻类绿藻中rRNA去除效率为80%,表明植物通用型riboPOOL可以应用于被子植物以外的植物门类。
riboPOOLs应用方向:
为了从含有多个物种的样本中去除rRNA,或为便于在包含多个物种的样本中使用一种试剂盒,可以使用组合riboPOOLs。这些可由客户指定以一定比例进行组合,至多可实现四种现成riboPOOLs的混合物。对高质量RNA样本的内部测试显示,组合riboPOOLs(人类/小鼠/泛原核生物)从人类、小鼠和大肠杆菌RNA中去除rRNA的表现与物种特异性 riboPOOLs效果类似。然而,siTOOLs不能确保组合riboPOOLs在所有样品类型中具有相似的去除效率,因为去除效率也高度依赖于样品的质量。
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