FGF1细胞因子

▍Backgound

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%-33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist.

▍Source

Recombinant Human FGF-1 is expressed from Escherichia coli. It contains AA Phe 16 - Asp 155. The protein has a calculated MW of 18.6 kDa. This protein carries a polyhistidine tag at the N-terminus.

Recombinant Human FGF-1 is expressed from Escherichia coli

▍Endotoxin

Less than 1.0 EU per μg by the LAL method.

▍AA Sequence

FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHA

EKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

▍Purity

Purity >95% as determined by SDS-PAGE.

▍Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

▍Reconstitution

Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

▍Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

▍SDS-PAGE

Human FGF-1, His Tag on SDS-PAGE under reducing (R) condition

Human FGF-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

厦门逸漠生物科技有限公司(Xiamen Immocell Biotechnology Co.,Ltd.)坐落于厦门国家留学创业园(翔安)产业园，是一家专注于细胞生物学领域新产品、新技术开发的高新技术企业。
公司拥有一支具有深厚造诣和丰富经验的高端技术人才，建设了高规格的研发实验室与生产车间，建立了规范的研发、生产、销售、客服管理体系。
公司与国内外多家医院、高等院校、科研院所、生物医药公司建立了良好的合作关系，赢得了良好的市场声誉和广泛的客户认可，将努力成为生命科学界可靠的合作伙伴和坚强的技术后盾。
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