代谢组

代谢组

 

Introduction

If genomics and proteomics in systems biology are compared to the science of exploring the causes of biological phenomena, metabolomics can be understood as the science of exploring the results of biological phenomena, that is, the science of directly studying the change law and function of all metabolites regulated by genes and proteins. On the one hand, metabonomics research can explore some new molecular markers. On the other hand, through linkage analysis with proteomics and genomics data, it can analyze the internal changes of organisms from both causes and results, so as to push the research of system biology to a higher level.

 

1 Technical characteristics  

1.1 GC-MS (gas chromatography) 

GC-MS is a classic technology in metabolomics research. It has the characteristics of mature and stable technology and high sensitivity. At the same time, due to its relatively perfect database, the qualitative analysis is more accurate and reliable. The disadvantages are mainly that the sample processing is relatively complex and it is difficult to determine the qualitative and quantitative analysis of substances that are not easy to be derived.

1.2 LC MS (liquid chromatography) 

The advantages mainly lie in simple sample preparation and pretreatment, good experimental repeatability, high resolution, wide separation and analysis range, while the disadvantages mainly lie in insufficient database perfection and relatively difficult qualitative analysis.

1.3 NMR (nuclear magnetic resonance) 

The biggest advantage is that it is non-destructive to the sample and non biased in the determination, that is, it is suitable for liquid samples such as blood, urine and body fluids, as well as solid samples such as tissues and organs. It has fast determination speed and can realize the dynamic monitoring of sample metabolome. The main disadvantage is low resolution.

 

2 Data analysis characteristics 

2.1 PCA (principal component) statistical analysis 

Principal component analysis is one of the commonly used analysis methods for metabolomic data. Through principal component analysis, we can understand the weight of different metabolites in distinguishing different samples, which is of great significance and value for the screening of different metabolites, especially important molecular markers.

2.2 PLSA Da (partial least squares discriminant analysis) statistical analysis 

Partial least squares discriminant analysis (PLS DA) is an important method of pattern recognition. This method can extract the simplified information from complex data to the greatest extent for analysis.

2.3 Metabolite function analysis 

Based on the screening of differential metabolites, the ultimate goal of metabolomics is to analyze the biological significance of metabolites. We can have a deeper understanding of the function and role of metabolites in organisms through statistical analysis such as PCA and clustering combined with pathway backtracking, bioinformatics analysis such as metabolites and protein / gene interaction.

五山（广州）生物医药科技有限公司成立于广州，先后在深圳、西安、南宁设立了办事处，是一家以健康为方向专注于核酸、蛋白质研究的生物医药企业。五山企业的骨干成员在科研市场和医疗健康领域沉淀有超过13年的研究、生产和市场经验。五山企业正开始逐步专注于微生物研究、诊断和治疗的赛道，努力建设一个优良的长青企业。
 

Wushan (Guangzhou) Biomedical Technology Co., Ltd established in Guangzhou, had set up branches in Shenzhen, Xian and Nanning, which is the life science company focusing on gene, protein and health. WuShan Company has more than 13 years experience in biological products, genomics, proteomics, medical health research fields. Comprehensive technical services basically cover the life science research fields. Since Wushan company establishment, it has become the strategic partner and preferred supplier of global well-known multinational companies and famous universities.

Wushan company has three brands: Wushan services, Wushan medicals and Wushan products. 

Wushan services provides bioinformatics analysis, high-throughput sequencing, proteome, metabolome and cell experiments for colleges, universities, scientific research institutions, hospitals and individuals.

Wushan medicals provides testing services.

Wushan products provides instrument & consumables including centrifuges, measuring cylinders, gloves, PCR tubes.

Wushan company commitment is consistent, which provides the best service portfolio to customers, including high-quality products, high-quality services, fast results and competitive.