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DESCRIPTION
Background:
Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family which are characterized by a shared structural motif, the EGF- like domain[1]. EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members[2]. EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals[3, 4]. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules[2]. Biological activities ascribed to EGF include epithelial development, angiogenesis, fibroblast proliferation, and colony formation of epidermal cells in culture.
FORMULATION AND STORAGE
Formulation:
The product is Lyophilized from a 0.22 μm filtered solution in PBS.
Shipping:
The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:
Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state;
12 months, -20 to -70 °C, under sterile conditions after reconstitution;
2 month, 2 to 8 °C under sterile conditions after reconstitution;
avoid repeated freeze-thaw cycles.
References:
1. Carpenter, G., et al., Epidermal growth factor. Biol Chem, 1990. 265(14): p. 7709-12.
2. Jorissen, R.N., et al., Epidermal growth factor receptor: mechanisms of activation and signalling. Exp Cell Res, 2003. 284(1): p. 31-53.
3. Gamett, D.C., et al., Secondary dimerization between members of the epidermal growth factor receptor family. J. Biol. Chem, 1997. 272: p. 12052.
4. Qian, X., et al., Heterodimerization of epidermal growth factor receptor and wild-type or kinase-deficient Neu: a mechanism of interreceptor kinase activation and transphosphorylation. Proc Natl Acad Sci U S A, 1994. 91(4): p. 1500-4.
Background:
Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family which are characterized by a shared structural motif, the EGF- like domain[1]. EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members[2]. EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals[3, 4]. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules[2]. Biological activities ascribed to EGF include epithelial development, angiogenesis, fibroblast proliferation, and colony formation of epidermal cells in culture.
技术参数
Source:
E.coli
Protein Construction:
A DNA sequence encoding the amino acids (Asn971-Arg1021) of human EGF (Accession Number: P01133) was expressed.
Synonyms:
hEGF; HOMG4; beta-urogastrone; epidermal growth factor; pro-epidermal growth factor; URG; Urogastrone
SPECIFICATIONS
Purity:
≥ 98%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.
Endotoxin Level:
<0.01 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:
5.95 kDa
SDS-PAGE:
8 kDa, reducing conditions
DATAE.coli
Protein Construction:
A DNA sequence encoding the amino acids (Asn971-Arg1021) of human EGF (Accession Number: P01133) was expressed.
Synonyms:
hEGF; HOMG4; beta-urogastrone; epidermal growth factor; pro-epidermal growth factor; URG; Urogastrone
SPECIFICATIONS
Purity:
≥ 98%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.
Endotoxin Level:
<0.01 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:
5.95 kDa
SDS-PAGE:
8 kDa, reducing conditions
FORMULATION AND STORAGE
Formulation:
The product is Lyophilized from a 0.22 μm filtered solution in PBS.
Shipping:
The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:
Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state;
12 months, -20 to -70 °C, under sterile conditions after reconstitution;
2 month, 2 to 8 °C under sterile conditions after reconstitution;
avoid repeated freeze-thaw cycles.
References:
1. Carpenter, G., et al., Epidermal growth factor. Biol Chem, 1990. 265(14): p. 7709-12.
2. Jorissen, R.N., et al., Epidermal growth factor receptor: mechanisms of activation and signalling. Exp Cell Res, 2003. 284(1): p. 31-53.
3. Gamett, D.C., et al., Secondary dimerization between members of the epidermal growth factor receptor family. J. Biol. Chem, 1997. 272: p. 12052.
4. Qian, X., et al., Heterodimerization of epidermal growth factor receptor and wild-type or kinase-deficient Neu: a mechanism of interreceptor kinase activation and transphosphorylation. Proc Natl Acad Sci U S A, 1994. 91(4): p. 1500-4.
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