Storage Buffer & Condition: PBS, pH 7.4, containing 0.02%
sodium azide as Preservative and 50% Glycerol.
Store at
-20°C.
Do not aliquot the antibody.
Recommended dilutions: WB: 1:5,000
Optimal dilutions should be determined by the end user.
Specificity: The T7 tag antibody can recognize T7 tag fusion proteins.
Background: The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein.
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