微管蛋白γ单克隆抗体

中文名称微管蛋白γ单克隆抗体

别    名Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1.  

研究领域细胞生物  神经生物学  细胞骨架  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号8D11

交叉反应Human, Mouse,  (predicted: Rat, )

产品应用WB=1:500-5000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量50kDa

细胞定位细胞浆 

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Gamma Tubulin: 

亚    型IgG

纯化方法affinity purified by Protein G

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Centrosome Marker

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.

The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

 

Function:

Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.

 

Subunit:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO.

 

Subcellular Location:

Cytoplasm, cytoskeleton, centrosome.

 

Tissue Specificity:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

 

Post-translational modifications:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

 

Similarity:

Belongs to the tubulin family.

 

SWISS:

P23258

 

Gene ID:

7283

 

Database links:

Entrez Gene: 27175 Human

 

Entrez Gene: 7283 Human

 

Entrez Gene: 103733 Mouse

 

Entrez Gene: 103768 Mouse

 

Entrez Gene: 252921 Rat

 

Entrez Gene: 680991 Rat

 

Omim: 191135 Human

 

Omim: 605785 Human

 

SwissProt: P23258 Human

 

SwissProt: Q9NRH3 Human

 

SwissProt: P83887 Mouse

 

SwissProt: Q8VCK3 Mouse

 

SwissProt: P83888 Rat

 

Unigene: 279669 Human

 

Unigene: 708059 Human

 

Unigene: 142348 Mouse

 

Unigene: 479145 Mouse

 

Unigene: 154431 Rat

 

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

结构蛋白（Structural Proteins）

gamma tubulin 微管蛋白是存于中心体的另一种微管蛋白, gamma微管蛋白对微管的形成具有重要作用。gamma微管蛋白通过与β-微管蛋白的相互作用帮助微管的成核反应(nucleation)。即在微管的组装中gamma微管蛋白先形成一个圆或形成钩环结构, γ微管蛋白的这种结构可指导微管蛋白二聚体结合上去并进行微管的组装。gamma微管蛋白在微管的组装中起关键作用。

产品图片

Sample: A549(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Gamma Tubulin (bsm-33313M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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