血管内皮生长因子受体1重组兔单克隆抗体

别    名 VEGF-R1; VEGFR-1; VEGF Receptor 1; FLT-1; vascular permeability factor receptor; vascular endothelial growth factor receptor; vascular endothelial growth factor receptor-1; fms-related tyrosine kinase 1; vascular endothelial growth factor/vascular permeability factor receptor; AI323757; FLT; FLT1; sFlt1; VGFR1_HUMAN; VGFR1_MOUSE.   

研究领域 肿瘤  细胞生物  免疫学  发育生物学  神经生物学  生长因子和激素  激酶和磷酸酶  细胞膜受体  细胞表面分子   

抗体来源 Rabbit 

克隆类型 Monoclonal 

克 隆 号 3A6 

交叉反应 Human, Mouse,  (predicted: Rat, ) 

产品应用 WB=1:1000-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 147kDa 

细胞定位 细胞浆 细胞膜 分泌型蛋白  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human VEGFR1:  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.

 

Function:

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiog

产品图片

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 4: U2os (Human) Cell Lysate at 30 ug Primary: Anti-VEGFR1 (bsm-52338R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 180 kD Observed band size: 190 kD Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFR1) Monoclonal Antibody, Unconjugated (bsm-52338R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

bio-equip.com