磷酸化叉头蛋白3A重组兔单克隆抗体

英文名称Phospho-FoxO3a (Ser253)

中文名称磷酸化叉头蛋白3A重组兔单克隆抗体

别    名FOXO3a(S253); FOXO3A (phospho S253); p=FOXO3A (phospho S253); FOXO1A (phospho S253); p-FOXO1A (phospho S253); AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box O3A; Forkhead box protein O3; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma-like 1; FOX O3A; FOXO2; foxo3; FOXO3_HUMAN; FOXO3A; MGC12739; MGC31925.  

产品类型磷酸化抗体 

研究领域肿瘤  免疫学  染色质和核信号  神经生物学  信号转导  转录调节因子  表观遗传学  

抗体来源Rabbit

克隆类型Monoclonal

克 隆 号25B3

交叉反应(predicted: Human, Mouse, Rat, )

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量71kDa

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated Synthesised phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253:AV(p-S)MD 

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

 

Function:

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

 

Subunit:

Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4/MST1, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Interacts with PIM1.

 

Subcellular Location:

Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors.

 

Tissue Specificity:

Ubiquitous.

 

Post-translational modifications:

In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

 

DISEASE:

Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.

 

Similarity:

Contains 1 fork-head DNA-binding domain.

 

SWISS:

O43524

 

Gene ID:

2309

 

Database links:

Entrez Gene: 2309 Human

 

Entrez Gene: 56484 Mouse

 

Entrez Gene: 294515 Rat

 

Omim: 602681 Human

 

SwissProt: O43524 Human

 

SwissProt: Q9WVH4 Mouse

 

Unigene: 220950 Human

 

Unigene: 338613 Mouse

 

Unigene: 24593 Rat

 

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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