细胞核因子/k基因结合核因子重组兔

研究领域 细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  转录调节因子   

抗体来源 Rabbit 

克隆类型 Monoclonal 

克 隆 号 3G1 

交叉反应 Human, Mouse,  (predicted: Zebrafish, ) 

产品应用 WB=1:1000-2000 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 61kDa 

细胞定位 细胞核 细胞浆  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human NFKB p65:  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Function:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-

产品图片

Sample: MCF-7(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Raw264.7(Mouse) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Spleen (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-NFKB p65 (bsm-52305R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 63 kD