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BL-C40TS-A2 ZAI AFM中对聚合物链小球的观测：
BL-AC10DS-A2 是一款快速扫描的可视频化的针尖，使用 HS-AFM的机台中可视化单个生物分子动力学的扫描；
大框架、活细胞 HS-AFM 成像
贴壁哺乳动物细胞通常跨越几十微米，因此标准 HS-AFM 系统只能对整个细胞表面的小子集进行成像。相比之下，商业 Bio-AFM 扫描仪提供覆盖整个细胞的最大扫描尺寸 (100 × 100 µm 2 )，但它们相对较低的扫描速度和在某些情况下有限的像素带宽阻止了以暂时解决动态膜所需的帧速率获取地形相关的细胞过程，例如胞内或胞吐事件，或皮质细胞骨架重排。使用我们的宽范围扫描仪，我们能够以20 秒/帧的速度对27 × 16 µm 2帧进行成像，该帧覆盖了活成纤维细胞外围的动态膜重塑区域（图 5和补充电影）5）。除了连续的膜下细胞骨架重塑外，我们偶尔还观察到在几个扫描帧中仍然可见的瞬时膜凹陷。基于它们的形态和瞬态性质，这些凹陷可能对应于网格蛋白介导的内吞凹坑的形成和最终膜覆盖。这种内吞凹坑的动态形成，以及导致其最终覆盖的肌动蛋白动力学的贡献先前已通过 HS-AFM 进行了详细研究，但仅限于相对扫描尺寸27 , 28. 相比之下，大型扫描仪游戏中时光倒流系列可以同时查看局部动态膜事件，例如与内吞摄取一致的圆形凹陷的瞬时形成，以及大范围事件，例如广泛的细胞骨架重排。因此，宽扫描仪 HS-AFM 可以以增强的帧速率提供整个细胞子区域的详细地形图像，能够在时间上解析细胞动力学。
Video clips: Morphological changes of plasmid DNA
Simple but strong desire of a biologist had moved us to make this ultra small cantilever for observing dynamic behavior of bio molecular samples in liquid. Bio sample is active in liquid, where cantilevers receive large damping force so that they vibrate slower rather than in air. Our challenge in the cantilever fabrication was maintaining resonance of the soft cantilever high even in liquid. The answer was ‘making it smaller’.
- 1. Ultra small cantilever for fast scanning in liquid
- 2. Carbon Nano Fiber probe for high resolution imaging ( BL-AC10FS-A2 )
More about BioLever fast
The ultra small cantilever sized in 9 x 2 µm shows high resonance at around 1.5 MHz in air and approximately 400 kHz in water while its spring constant remain small around 0.1 N/m.
The resonance in water is 10 times higher than that of ‘BioLever mini’, or BL-AC40TS-C2 , so that it contributes to achieve imaging rate of 1 frame per second or faster for 100 x 100 pixcels.
Its spring constant is small in the same range as ‘BioLever mini’, so that it allows the probe gently touch to the bio sample surface during the scanning.
A CNF probe, small fibril with a tip radius less than 10 nm, grows at the end of the triangular portion of bird-beak-like cantilever. CNF probe improves quality of AFM images.
BL-AC10DS-A2 (BioLever fast without CNF probe) is available as before. They can be used for imaging as it is, and also be used as a basic cantilever for modifying, e.g. depositing EBD (electron beam deposition) tip.
A corner of the triangular protrusion at the end of the bird-beak-like cantilever behaves as a probe tip. The protrusion is a plate-like, 0.8 µm in length and its apex is 24 nm in radius ( nominal ).
In taking AFM movies for observing dynamic behavior of bio samples like DNA strands, the protrusions with relatively large tip-apex often result less scratched lines in the images.
For high resolution imaging with high speed AFMs, EBD tips have been used. It works better than CNF probe in some cases, e.g. observation of relatively large samples. EBD tip with desired length and direction can be made by controlling electron beam irradiation.
实际应用：
2.https://www.nature.com/articles/s42003-021-02388-4 BL-AC10DS -A2 病毒因子RNA 合成
3.https://www.nature.com/articles/srep07818 BL-AC10DS-A2 机械震荡
4.https://www.nature.com/articles/s41467-021-25023-6 BL-AC40TS-C2 DNA记忆堆栈
5.https://www.nature.com/articles/ncomms11566 BL-AC40TS-C2 水凝胶AFM纳米探针
6.https://www.nature.com/articles/s41598-021-91371-4 BL-AC40TS-C2 SARS-CoV-2 包膜病毒，COVID-19冠状病毒病 天然传染性和灭活分析
Dimensions of levers and chip (substrates)
| BL-AC10FS-A2 / BL-AC1DS-A2 | |
|---|---|
| L (µm) | 9 |
| W (µm) | 2 |
| t (µm) | 0.13 |
上海展悦电子科技有限公司
郑小姐
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