敏狄康小动物肾功能监测及智能分析系统(TGFR),由 MediBeacon 公司研发生产,采用先进的经皮测量荧光技术,通过静脉注射荧光标记的外源性示踪剂,经皮、连续地实时监测皮下组织荧光强度变化来记录完整清除曲线,从而计算半衰期(T1/2)和肾小球滤过率(GFR),无需采血采尿,测量过程中动物保持清醒自由活动状态,对动物无损伤刺激,避免了注射麻醉引起的并发症,更接近生理值,也可进行实时干预。
实现肾损伤早发现 跟踪肾功能长期变化
目前,临床前动物实验针对急性肾损伤或慢性肾病的肾功能检测方法主要通过对血液和尿液中的内源性标志物如肌酐等进行检测。敏狄康®TGFR系统具有独特的科技创新性,可以对同一只动物进行多次测量,相比于内源性标志物,能够在早期观察到动物的肾损伤,在急性或慢性疾病条件下进行一系列的肾功能监测,并跟踪肾脏功能的长期变化。
革新传统检测方法 肾功能测定金标准
作为目前全球临床前唯一的可实现经皮、精准、简便的肾功能监测的科技创新分析系统,敏狄康®TGFR系统受到了众多专家的认可。德国慕尼黑大学肾内科博士后Julian Aurelo Marschner称,传统的采血测定方法属于侵入性测定,存在监测困难、操作繁琐、无法连续监测和结果不宜重复的缺点;而敏狄康®TGFR系统可靠且稳健,信号非常敏感,内置的软件可以帮助研究者高效分析数据,堪称肾脏病学领域革新,有机会取代传统测量方法,成为小动物肾功能测定新的金标准。
操作方式简便快捷 技术人员轻松上手
加州大学戴维斯分校Lauren Lopez博士称,敏狄康®TGFR系统在硬件配置和设计方面充分考虑了使用者的习惯,整个过程简单便捷,无需特殊培训即可轻松上手,一名熟练的实验技术人员在一天正常工作时间里可以完成30~40只小鼠的敏狄康®TGFR监测。
Cardiovascular
[1] Hu C, et al. Renomedullary interstitial cell endothelin a receptors regulate BP and renal function.
J Am Soc Nephrol, 2020. doi:
10.1681/ASN.2020020232.
[2] Shi C X, et al. Crystal clots as therapeutic target in cholesterol crystal embolism.
Circ Res, 2020, 126. doi: 10.1161/CIRCRESAHA.119.315625.
[3] Gupta N, et al. Targeted inhibition of gut microbial TMAO production reduces renal tubulointerstitial fibrosis and functional impairment in a murine model of chronic kidney disease.
Arterioscler Thromb Vasc Biol, 2020. doi: 10.1161/atvbaha.120.314139.
Diabetes
[1] Wang F, et al. Soluble (pro)renin receptor treats metabolic syndrome in mice with diet-induced obesity via interaction with PPARγ.
Am Soc Clin Investig, 2020. doi: 10.1172/jci.insight.128061.
[2] Geng X Q, et al. The urea transporter UT-A1 plays a predominant role in a urea-dependent urine-concentrating mechanism.
J Biol Chem, 2020. doi: 10.1074/jbc.RA120.013628.
[3] Sukumaran V, et al. Liraglutide treatment improves the coronary microcirculation in insulin resistant zucker obese rats on a high salt diet.
Cardiovasc Diabetol, 2020. doi: 10.1186/s12933-020-01000-z.
Immunology & Inflammation
[1] Shaver C M, et al. Cell-free hemoglobin augments acute kidney injury during experimental sepsis.
Am J Physiol Renal Physiol, 2019, 317: F922–F929.
[2] Sims C R, et al. Rolipram improves outcome in a rat model of infant sepsis-induced cardiorenal syndrome.
Front Pharmacol, 2017, 8: 237.
Metabolism & Regulation
[1] Katsyuba E, et al. De novo NAD+ synthesis enhances mitochondrial function and improves health.
Nature, 2018, 563: 354–359.
[2] Kasztan M, et al. Long-term endothelin-a receptor antagonism provides robust renal protection in humanized sickle cell disease mice.
J Am Soc Nephrol, 2017, 28: 2443–2458.
Renal
[1] Geng X Q, et al. The urea transporter UT-A1 plays a predominant role in a urea-dependent urine-concentrating mechanism.
J Biol Chem, 2020. doi: 10.1074/jbc.RA120.013628,
[2] Zhang X G, et al. Renal fibrosis is significantly attenuated following targeted disruption of Cd40 in experimental renal ischemia.
J Am Heart Assoc, 2020, 9: e014072.
[3] Cippà P E, et al. A late B lymphocyte action in dysfunctional tissue repair following kidney injury and transplantation.
Nat Commun, 2019, 10: 1157.
Toxicology
[1] Fu Y, et al. Chronic effects of repeated low dose cisplatin treatment in mouse kidneys and renal tubular cells.
Am J Physiol Renal Physiol, 2019, 317. doi: 10.1152/ajprenal.00385.2019.
[2] Benjamin A, et al. Renal studies in safety pharmacology and toxicology: a survey conducted in the top 15 pharmaceutical companies.
J Pharmacol Toxicol Methods, 2015, 75: 101–210.
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