重组SARS-CoV S-RBD(His标签)(>95%)

特别提示：包括重组SARS-CoV S-RBD(His标签)在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组SARS-CoV S-RBD(His标签)
英文名称：SARS-CoV S-RBD Protein(His Tag)
产品货号：Q10201
产品规格：100μg

纯度：> 95% as determined by SDS-PAGE
内毒素：<1.0 EU per μg of the protein as determined by the LAL method
GenBank No.：AAX16192.1
表达宿主：Baculovirus-Insect Cells
种属：SARS
预测N端：Arg306

蛋白构建：
A DNA sequence encoding the receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with a C-terminal polyhistidine tag.

分子量：
The recombinant receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike comprises 233 amino acids and has a predicted molecular mass of 26.5kDa.The apparent molecular mass of the protein is approximately 35.1kDa in SDS-PAGE under reducing conditions.

制剂：
Lyophilized from sterile 20mM PBS，300mM NaCl，10% glycerol，pH7.0

复溶：
A hardcopy of COA with reconstitution instruction is sent along with the products.Please refer to it for detailed information.

稳定性&储存条件：
Samples are stable for up to 12 months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃.It is recommended that the protein be aliquoted for optimal storage.Avoid repeated freeze-thaw cycles.

背景资料：
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell.Known receptors bind S1 are ACE2,angiotensin-converting enzyme 2;DPP4,dipeptidyl peptidase-4;APN,aminopeptidase N;CEACAM,carcinoembryonic antigen-related cell adhesion molecule 1;Sia,sialic acid;O-ac Sia,O-acetylated sialic acid.The spike is essential for both host specificity and viral infectivity.The term "peplomer" is typically used to refer to a grouping of heterologous proteins on the virus surface that function together.The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process.It"s been reported that SARS-CoV-2 (COVID-19 coronavirus,2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.The spike protein is a large type I transmembrane protein containing two subunits,S1 and S2.S1 mainly contains a receptor binding domain (RBD),which is responsible for recognizing the cell surface receptor.S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses,as well as protective immunity.The main functions for the Spike protein are summarized as:Mediate receptor binding and membrane fusion;Defines the range of the hosts and specificity of the virus;Main component to bind with the neutralizing antibody;Key target for vaccine design;Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD),leading to a higher mortality rate.

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关注重组SARS-CoV S-RBD(His标签)(>95%)，SARS Spike RBD Protein，重组SARS病毒受体结合域，重组His标签，SARS-CoV S-RBD Protein(His Tag)，重组SARS-CoV S-RBD，重组SARS-CoV S1-RBD，重组SARS-CoV S-RBD Protein(His Tag)的同时，为您推荐更多您可能感兴趣的产品：



名称：重组小鼠趋化因子CCL2(MCP-1)
货号：JN0331
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备，目的基因编码的Gln24-Arg96表达纯化而来。

CCL2/MCP-1质量控制：＞95%(还原性SDS-PAGE)。

CCL2/MCP-1制剂：冻干品

CCL2/MCP-1保存：
冻干蛋白可在-20℃长期保存，室温也可稳定保存3周。
复溶蛋白溶液可在4～7℃保存2～7天。
复溶后建议分装冻存在-20℃，可保存3个月，应避免反复冻融。

CCL2/MCP-1复溶：
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存，避免反复冻融。

关于CCL2/MCP-1：
C-C motif chemokine 2 (CCL2) is a member of the C-C or β chemokine family. Mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source. In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2. Additionally, it promotes Th2 polarization in CD4+ T cells. CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.

北京百奥莱博科技有限公司一家专注于生命科学研究领域的生物技术公司，坚持“质量至上，服务为本，全心全意助力生命科学”的理念，为广大用于提供稳定、可靠、专业的产品和服务支持，产品线包括分子生物学，细胞生物学，免疫检测，抗体，重组蛋白，血液制品等系列。“忙忙碌碌的平凡，铸就事业的不凡”。让我们携起手来，共同创造生命科学的蓬勃发展。