PeproTech重组人Tissue Factor

Tissue factor is a transmembrane glycoprotein of the cytokine receptor superfamily that acts as a receptor for coagulation factor VII (fVII) to trigger initiation of the coagulation cascade in response to vascular injury. Expression of tissue factor occurs constitutively within most extravascular and perivascular cells and at high levels within critical organs and tissue. Tissue factor is not normally expressed freely on the surface of circulating blood cells due to its pro‐coagulant effect, but is instead stored on the surface of mononuclear and endothelial cells in microparticles that can shed into circulation in response to vascular injury, pro‐inflammatory cytokines, or microbial ligands. Tissue factor can also be shed into circulation by cancer cells where its expression in a number of cancer types has been linked to tumor progression, metastatic potential, thrombosis, and angiogenesis. Expression of tissue factor has been shown to be inducible by select cytokines in a number of cell types, including
IL‐1β and TNF‐α in vascular endothelial cells and macrophages, and TNF‐α, IL‐6, and FGF‐Basic in monocytes, among others. PeproTech’s CHO cell‐derived Recombinant Human Tissue Factor is a glycoprotein homodimer comprised of the 227‐amino‐acid length monomer containing a C‐terminal His‐Tag. It has a calculated molecular weight of approximately 25.8 kDa, and as a result of glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 40‐50 kDa by SDS‐PAGE gel.

Source:CHO cells

Synonyms:TF, TF1, Coagulation factor III, factor III, F3, Thromboplastin, CD142

AA Sequence (monomer):SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GGEKGEFREH HHHHHHH

Purity:≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII.

Calculated Molecular Weight:51.6 kDa

Accession Number:P13726

Gene ID:2152
Country Of Origin: USA

Not for human use.

PeproTech Inc. 创立于1988年，始终致力于为生命科学研究开发重组蛋白及相关产品，如今已成为能提供多种高质量细胞因子和重组蛋白，相应的单克隆、多克隆和生物素标记抗体，以及ELISA试剂盒的世界领导者。PeproTech Inc.的产品涵盖免疫调节蛋白、白介素、集落刺激因子、生长因子、化学趋化因子、干扰素、脂联素、成纤维生长因子、转化生长因子、肿瘤坏死因子、神经营养素和防御素等诸多生命科学研究领域中重要的生物活性分子。

为跟踪当今科研发展、满足日益增长的需求，PeproTech Inc.研制出无动物成分的重组蛋白(animal-free recombinant proteins)。有效地避免了动物源性污染引发潜在问题的可能性，从而可以应用于临床前研究和细胞治疗。此外，PeproTech Inc.最新还开发出无动物成分的培养基添加物试剂盒(animal-free Media Supplement Kits)。这些试剂盒经改良设计，其中的无血清组分对多种哺乳动物细胞的生长有更好的维持能力。

PeproTech Inc.的产品自2000年进入中国市场以来，以其优良的品质和合理的价格迅速得到国内各重点研究和实验机构的认可和青睐。鉴于中国经济发展的良好态势和中国生物医药研发投入的巨额增长，PeproTech Inc.于2009年10月在中国苏州设立代表机构，意为中国的广大用户提供更好的支持和服务，并助中国的科研和产业开发一臂之力。