Introduction
RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
Product Features
- High sensitivity (pg/mL to ng/mL levels)
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Test 16 samples on each slide
- Data analysis software provides one-step computation
Species Detected
Mouse
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
200
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广州瑞博奥生物科技有限公司(RayBiotech, Inc. Guangzhou)是一家集生物高科技产品研究、生产、销售于一体的外资企业,由国际公认的蛋白质芯片专家黄若磐博士创办。黄若磐博士同时在美国亚特兰大科技园拥有蛋白质芯片先驱公司RayBiotech, Inc.已有长达10多年之久的品牌发展。 广州瑞博奥生物科技有限公司公司注册资金800万美元,总投资5000万美元,获得2009年度广州政府首批科技领军人才项目资助,在广州开发区拥有20亩的在建厂房。
广州瑞博奥生物科技有限公司拥有抗体芯片平台、 蛋白质芯片平台、ELISA/EIA试剂盒平台、免疫和诊断试剂平台、芯片定制服务平台、样品检测服务平台、芯片数据分析服务平台。公司涉及的产品主要有夹心法抗体芯片、标记法抗体芯片、定量抗体芯片、磷酸化抗体芯片、蛋白质芯片近万种,并且提供生物标志物和药物开发服务。公司拥有先进的技术优势、遍布全球的销售网络以及世界各国众多科学家客户中很高的产品美誉度。许多发表在Nature、Nature Medicine、Cell、PNAS、Lancet 等世界顶级期刊上的文章中均使用了瑞博奥的产品,并且对公司芯片技术和产品的质量都给予了肯定和很高的评价。
Raybiotech 是美国蛋白芯片公司的领头羊,其产品涵盖以下领域:
原料类:3637种重组蛋白(recombinant protein);1081 种多肽(peptide);7881种抗体(antiboday);酶联免疫试剂盒:500种ELISA试剂盒,更多试剂盒正在推出;24种cell-based ELISA;10种EIA 试剂盒(检测多肽类);
蛋白芯片:52种Quantibody Array;19 种Label-Based Array(L-series);12 种Phosphorglation Array;11种Tissue Array;144种Antibody Array;还可以定制根据您的兴趣来定制芯片;
Nature, Nature Medicine, Cell, PNAS, Lancet 等世界顶级期刊上数千篇文章中均使用了RayBiotech的产品。
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