RFect siRNA转染试剂/小核酸转染试剂

货号	规格（ml）	目录价（元）
11011	0.5	1,080
11012	1	1,830
11013	1.5	2,590

产品简介

RFect小核酸转染试剂是知名科学家崔坤元博士领导我公司研发团队在美国西雅图实验室研发成功的一种新型的小核酸转染试剂。RFect可用来转染siRNA、miRNA mimics、miRNA inhibitor 等200bp以内的小分子RNA，转染细胞包括绝大多数贴壁生长的细胞，如一般细胞株、肿瘤细胞株等。目前，无论国外还是国内，转染试剂的主要成分均为脂质体或聚乙烯亚胺(Polyethylenimine, PEI)，这两种成分都具有很大的细胞毒性，并且转染效果不好。RFect采用新型的动物源性的纳米材料，毒性很低并且拥有非常高的转染性能。与其它品牌的小核酸转染试剂相比，RFect的细胞转染阳性率一般在90%以上，Lamin A/C 基因抑制效率在95%以上，而其它品牌转染试剂的细胞转染阳性率一般不超过70%，Lamin A/C 基因抑制效率不超过75%。RFect细胞毒性很低，转染细胞死亡率不到10%，而其它品牌试剂的转染细胞死亡率一般在30%以上。血清对转染效果没有影响，不必刻意添加或更换培养液。本产品适用于细胞株转染，原代细胞转染，请选择RFectPM小核酸转染试剂。

产品特点

·细胞转染性能：细胞转染阳性率高达90%以上，基因敲除效果明显，A549细胞Lamin A/C 基因敲除效率在95%以上；

· 极低的细胞毒性：转染细胞死亡率不到10%，大大降低了因细胞毒性对实验结果的影响；

· 转染细胞范围广，绝大多数贴壁细胞株都能获得比较理想的转染结果。
 

适用细胞

293T（人肾上皮细胞），A549（人肺癌细胞），HEK 293（人胚肾细胞），HeLa（人宫颈癌细胞），MCF-7（人乳腺癌细胞），HepG2（人肝肿瘤细胞），HT-1080(人纤维肉瘤），HLF-a（人肺细胞），Huh-7（人肝癌细胞），KB（人口腔表皮样癌细胞），PC3（人前列腺癌细胞），BRL-3A（大鼠正常肝细胞），DU 145（人前列腺癌细胞），Hepa 1-6（小鼠肝癌细胞），OVCAR3（人卵巢癌细胞），NCaP-FGC（人前列腺癌细胞），C2C12（成肌细胞），MDCK（犬肾细胞），CHO-K1，islk.219细胞（人肺癌细胞），HT-29（人结肠癌细胞），NCI-H1688（人典型小细胞肺肿瘤/癌细胞），RKO结肠癌细胞，Vero（非洲绿猴肾细胞），COS-1（非洲绿猴肾成纤维细胞），COS7-L（非洲绿猴肾成纤维细胞），COS-7（非洲绿猴肾成纤维细胞），SK-BR3（人乳腺癌细胞），Hepa 1cLc7（鼠肝癌细胞），Daoy（人髓母细胞瘤细胞），DBTRG-05MG（人脑胶质母细胞瘤细胞），KLN 205（鼠肺磷癌细胞），Neuro-2a（小鼠神经瘤母细胞），DI-TNC1（大鼠脑间质细胞）等。
 

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常州百代生物科技股份有限公司（BIOG,  Changzhou Bio-generating Biotechnology Corp）由留美归国科学家发起设立，主要从事核酸递送技术及相关产品的研发与生产。公司是国内较早从事核酸递送技术及相关产品研发的公司，现拥有三大核酸递送技术平台，包括siRNA纳米胶束递送技术、mRNA VLP技术和mDNA脂质囊球递送技术，是国内拥有核酸递送核心技术较多的公司。在相关技术的基础上，公司开发了一系列产品，包括Rfect小核酸转染试剂、RfectPM原代细胞小核酸转染试剂、RFectSP悬浮细胞小核酸转染试剂、Elite Fect-I小核酸活体组织转染试剂、Elite Fect-II小核酸体内转染试剂、质粒DNA转染试剂和人工装配病毒样颗粒等。这些产品已在科研领域广泛应用，其核酸递送性能得到了市场的广泛认可和充分验证。特别是Rfect系列转染试剂，siRNA递送效率和细胞毒性均显著优于权威品牌，已成为国内siRNA递送载体中的知名品牌。与之相关的技术，已获国内发明专利授权。

除核酸递送技术外，我们还拥有核酸检测相关的核心技术。公司的sf-qPCR技术为专利技术，泌尿生殖道感染核酸检测技术获国家发明专利授权。我们的核酸提取试剂采用了独特的细胞裂解技术，核酸提取得率高并且提取时间大幅缩短，BIOG血液核酸提取试剂、血浆游离RNA提取试剂、拭子DNA提取试剂、预装拭子核酸提取试剂已被市场广泛认可并享有较高的美誉度。公司的热启动Taq酶，具有良好的检测灵敏度、酶动力活性和反应稳定性，非常适合于各类荧光定量PCR实验和高质量PCR试剂盒的研制，即使与著名品牌的热启动Taq酶相比，我们的产品也毫不逊色。此外，公司的BIOG系列荧光定量PCR试剂、宠物系列核酸检测试剂、自动核酸提取仪等均享有较高的市场认可度。