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chromotek公司U2OS-CCC-TagGFP/现货
英文名称:U2OS-CCC-TagGFP总访问:586
国产/进口:进口半年访问:3
产地/品牌:德国/chromotek产品类别:细胞生物学试剂
规       格:U2OS-CCC-TagGFP 最后更新:2025-2-19
货       号:
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 U2OS-CCC-TagGFP

  

 

  Description

  U2OS-Cell Cycle Chromobody®-TagGFP

  U2OS cell line stably expressing Cell Cycle Chromobody® fused to the fluorescent protein TagGFP2

  Specificity

  VHH antibody fragment against cell cycle marker PCNA (proliferating cell nuclear antigen)Actin-Chromobody®

  Live cell imaging of U2OS-Cell Cycle Chromobody®-TagGFP2

  

 

  For live cell imaging cells were seeded in ibidi µ-Slides. Time series was acquired on a spinning disk microscope (UltraVIEW VoX, PerkinElmer) equipped with a 63x/1.4 NA Plan-Apochromat oil immersion objective and a humidified, temperature- and CO2-controlled live cell chamber. TagGFP2 was excited with a 488 nm laser-line for typically between 100-500 ms/ image with laser intensity set to max. 25%. Confocal image z-stacks of living cells were recorded with a frame size of 1000x1000 pixels, a pixel size of 100 nm and a z-step size of 400 nm every 30 minutes. Images shown are maximum intensity projections of few mid-z-sections.

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