●系统的工作需要Gene Pulser 核心主件和可选择的三种模块共同完成,
用户根据实验室的需要来选择不同的组件,用于原核和真核细胞的转染
●脉冲增强控制器模块用于细菌和酵母的转化,它能提供高电阻控制,有
效地 转移任何大小或复杂度的DNA或蛋白质,同时提供最大限度的样品保
护功能
●电容扩充器模块用于哺乳动物细胞、胚胎细胞或植物原生质体的穿也,
具有广泛、精确的电容(达到3275μF),确保 用更大的电容进行低电压
转化最敏感的真核细胞或细胞块
●RF模块,与核心主件连用时可输出Bio-Rad专利的脉冲,称Flexwave技术,
样品被方波或射频波形电激。此技术有利于保持转化时细胞的高存活率
●先进的电路系统设计,可通过严格的电容、电阻和电压控制来实现精确的
脉冲传输,系统的弧光保护可以避免仪器损伤,每个模块有独特的连接电缆,
防止错误连线
The Gene Pulser Xcell™ system is a modular electroporation system for transfecting every cell type. The system includes a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE module) and the pulse controller (PC module).
Features and Benefits
- Universal electroporation — transfects all cell types, from primary and stem cells to bacteria and yeast
- Preset protocols — include the most common mammalian and bacterial cell types
- Flexibility — choice of programs for preset protocols, optimization protocols, manual operation, and/or user protocols
- Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
- Data management — enables storage and recall of parameters used in the previous 100 experiments, useful for troubleshooting
- Reproducibility — uses PulseTrac™ circuitry and arc-protection features to ensure reproducibility and sample protection
System Options
- Gene Pulser Xcell total electroporation system (catalog #165-2660) — the complete electroporation system for transfection of both eukaryotic and prokaryotic cells; includes both the CE module, and the PC module
- Gene Pulser Xcell eukaryotic electroporation system (catalog #165-2661) — for the electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts; includes the CE module
- Gene Pulser Xcell microbial electroporation system (catalog #165-2662) — for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume; includes the PC module
More Information
- Features modifiable delivery parameters including time constant, actual voltage applied, pulse interval, and pulse time, depending on the waveform (exponential or square) chosen
- Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol
- Compatible with 0.1 cm, 0.2 cm, and 0.4 cm Gene Pulser®/Micropulser™ electroporation cuvettes
- Compatible with any electroporation buffer, including Bio-Rad's Gene Pulser electroporation buffer
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