mobitec的巨大芽孢杆菌表达系统

 mobitec的巨大芽孢杆菌表达系统

hp-Vector表达系统megaterium芽孢杆菌

hp-Vector Expression Systems for Bacillus megaterium

Features:

       High performance vectors with optimized sequence

       Protein yield up to 10 times enhanced compared to protein expression with basic plasmid

       Plasmids with established MCS

       Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)

Secretion with LipA or YocH signal peptide up to 9 fold increased

optimized high performance (hp) vectors for Bacillus megaterium!

MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.

With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.

Versatile system with a wide range of vectors

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

B. megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Order Information, shipping and storage

Please click on the respective order # for further details.

order# description amount

BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1)

Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl

BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl

BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg

BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg

BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg

BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg

BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg

shipped at RT, protoplasts shipped on dry ice

store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C

All vectors are E. coli / B. megaterium shuttle vectors

Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.

北京毕特博生物技术有限责任公司

全国热线：400-833-9299

电话： 13366202681 张钰

QQ：1767180321

传 真：010-62015131

邮箱：zym_ly@126.com

北京毕特博生物技术有限责任公司（Beijing Bitab Biotech Co.,Ltd）是一家成立于2001年(始于1994年的毕龙转基因)，经营生命科学用品的高科技企业。全国最早的生物代理公司之一，多个国际知名品牌国内第一家代理。公司主要代理各大国际著名生物公司的试剂、仪器、设备和耗材等。同时提供各类生物技术服务、协助科研设计和数据处理、组织国内和国际学术会议、提供相关网络服务等。

MP Biomedicals生物医学公司是位于美国加利福尼亚州的知名生命科学和生物技术厂商。它致力于生命科学领域的基础研究和探索。目前它提供55000多种产品，是世界上能全面提供生命科学产品和临床诊断产品的唯一几家厂商之一。

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Alexis公司是一家集生命科学试剂研发、生产和销售于一体的综合性私营企业，它已经在世界范围内成功建立了稳定的市场达10年之久，具备超强的管理体系和电子商务能力。Alexis公司总部在美国，并在英、德、瑞士均设有分部，便于试剂的运输和调配。此外，该公司还拥有资深的生物领域专家做为技术支持，并且代理其它品牌的一些生命科学领域主要研究方向的产品。

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ICN生物医学化学药品公司从1960年成立以来，随着其生物医学科研产品的开发和扩展，现在已经成为整个世界上生物技术科研产品和医学临床诊断产品的身居主导地位的供应者。ICN现在供应数千种化学药品、化学试剂、实验设备和诊断器械，能够满足整个生物技术领域的全部需求。