ER-receptor

Mouse mAb to ER-receptor
Clone AER314
lsotype Mouse IgG1-lambda
Source
Estrogen receptor antigens purified from calf uterus were injected subcutaneously in Balb/C mice. Two more doses, emulsified with Freund''s incomplete adjuvant were injected at 15-day intervals. Two additional doses were injected intraperitoneally before the mice were killed. Spleen cells were fused with Sp2/000-Ag14 mouse mycloma cells.
Specifications
The human estrogen receptor has a Mw of 67 kDa and consists of 6 functional domains (domain A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone- binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor. The mAb A-ER 314 is binding the human estrogen receptor in the B domain (aa 120-170).
Applications
The mAb AER 314 is applicable for staining of mouse and human frozen tissue sections and paraffin sections. It specifically stains the nuclear area of the cell.
Frozen section +
Paraffin sections +
Flow cytometry
ELISA +
Blocking
Immuno-precipitation +
Western blotting +

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