不同的细胞培养条件对细胞群体分布的影响
应用实例列举(详情请向客服索取)
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细胞死亡预测
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外周血单核细胞计数
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Ehrlichiosis小鼠模型中脾细胞状态监测
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Stem cell 分化状态监测
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牛奶中体细胞监测
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酿酒业酵母菌的计数分析
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微球的计数
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海水中藻类研究
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真菌孢子的检测(研究领域:啤酒业。农作物白粉病)
一、工作原理
库尔特原理:V=IR
V(电压),I(电流),R(电阻值)
i. Scepter感应器吸入精确的样本体积。
ii. 细胞穿过感应器小孔时,电阻值增加。电阻值的增加导致电压的增加
iii. 每个细胞经过时引起的电压的增加作为一个脉冲被记录下来
iv. 用直径和细胞数目绘制直方图,脉冲的大小代表细胞的大小,脉冲的数目代表细胞的个数
二、结果显示
三、Sensor的选择
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40 µm 感应器
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60 µm 感应器
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样本的浓度范围
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50,000-1,500,000 个/毫升
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10,000-500,000 个/毫升
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颗粒的测定直径
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3-17 µm
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6-36 µm
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颗粒的推荐可测直径
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4-13 µm
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8-25 µm
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相应的产品货号
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50包装: PHCC40050
500包装: PHCC40500
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50包装: PHCC60050
500包装: PHCC60500
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四、数据分析
数据可直接在Scepter计数器上进行分析:
手动/自动设门:对您感兴趣的细胞群体进行统计计数
通过设门可以排除非完整细胞的碎片,或非目的细胞群
采用相同的设门参数对平行样品进行统计分析,可减少不同样品的结果偏差。
五、关于精确度
为什么Scepter的精确度会被认可?
1、Scepter统计的细胞数量远多过其他计数方法
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Scepter会统计吸入的50ul中的所有细胞,而其他依靠可视原理的细胞统计的样本体积远低于Scepter,统计的细胞数量更是少之又少。
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对于浓度为100000个/ml的样品所统计的细胞数量
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Scepter=5000个细胞
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血球计数板:0.1ul
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血球计数板=10个/方格
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其他可视计数系统:0.4ul
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可视计数系统=40个细胞
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2、Scepter对低浓度细胞样品的统计仍然具有高精确度
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对样品中所有细胞进行统计,使得Scepter对低浓度样品仍然保持高准确度
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包括血球计数板在内的可视计数方法只有对较高浓度的样品计数时,才能具有一定的精确度
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3、Scepter对小直径的细胞计数仍然具有高精确度
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Scepter的阻抗检测原理,以及对细胞体积进行直接测量的方式,使得对较小细胞的计数依然准确
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可视计数方法对<6uM直径的细胞很难统计(难将其与细胞碎片等颗粒区分开)
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六、延保计划
Scepter享有一年的保质期,如果购买 保质期延长计划,则享有二年的保质期。
七、订购信息
具体产品价格请询问当地销售人员
八、发表文献
IL-23 Dampens the Allergic Response to Cryptococcus neoformans through IL-17 -Independent and -Dependent Mechanisms
Szymczak WA, Sellers RS, Pirofski LA (2012) The American Journal of Pathology. Epub Feb
15.
PMID:22342846
Application of Scepter: Cell counting (primary mouse lung cells)
Retinoic acid-induced differentiation increases the rate of oxygen consumption and
enhances the spare respiratory capacity of mitochondria in SH-SY5Y cells.
Zhiyin Xun, Do-Yup Lee, James Lim, Christie A. Canaria, Adam Barnebey, Steven M.
Yanonne, Cynthia T. McMurray (2012) Epub Feb 7.
PMID: 22336883
Application of Scepter: Cell counting (SH-SY5Y cells, human neuroblastoma cell line)
NAD(P)H:quinone oxidoreductase 1 protects lungs from oxidant-induced emphysema in
mice.
Erin N. Potts-Kant, Zhuowei Li, Robert M. Tighe, James Y. Lindsey, Benjamin W. Frush, W.
Michael Foster, John W. Hollingsworth (2012) Free Radical Biology & Medicine. 52:705-15.
PMID: 22198263
Application of Scepter: Cell counting (Bronchoalveolar lavage fluid derived cells)
Does NAD(P)H oxidase-derived H2O2 participate in hypotonicity-induced insulin release
by activating VRAC in β-cells?
Crutzen R, Shlyonsky V, Louchami K, Virreira M, Hupkens E, Boom A, Sener A, Malaisse WJ,
Beauwens R (2012) Pflugers Arch. 463:377-90.
PMID: 22089811
Application of Scepter: Cell counting, Cell diameter monitoring (BRIN-BD11 cells, a insulin
secreting beta-cell line; primary rat islet cells)
Anti-inflammatory effects of benfotiamine are mediated through the regulation of the
arachidonic acid pathway in macrophages.
Mohammad Shoeb, Kota V. Ramana (2012) Free Radical Biology & Medicine. 52:182-90.
PMID: 22067901
Application of Scepter: Cell counting (RAW264.7 cells, a mouse leukaemic monocyte
macrophage cell line)
Reduction of infarct size by intravenous injection of uncultured adipose derived stromal
cells in a rat model is dependent on the time point of application.
van Dijk A, Naaijkens BA, Jurgens WJ, Nalliah K, Sairras S, van der Pijl RJ, Vo K, Vonk AB,
van Rossum AC, Paulus WJ, van Milligen FJ, Niessen HW (2011) Stem Cell Research. 7:219-29.
PMID: 21907165
Oncogenes induce senescence with incomplete growth arrest and suppress the DNA
damage response in immortalized cells.
Michael Y. Sherman, Le Meng, Martha Stampfer, Vladimir L. Gabai, Julia A. Yaglom (2011)
Aging Cell. 10:949-61.
PMID: 21824272
Application of Scepter: Cell counting for growth curve monitoring (human mammary epithelial
cells; MCF10A cells, a non-tumorigenic epithelial cell line)
Synthetic Reversal of Epigenetic Silencing.
Haynes KA, Silver PA (2011) J Biol Chem. 286:27176-82.
PMID: 21669865
Application of Scepter: Cell counting (HEK293 cells)
Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and
Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells.
Patnaik SK, Kannisto E, Mallick R, Yendamuri S (2011) PLoS One. 6:e22379.
PMID: 21789255
Application of Scepter: Cell counting (A549 cells, human lung adenocarcinoma; BEAS-2B cells,
a normal human bronchial epithelial cell line; TLA-HEK293T cells, a human embryonic kidney
cell line)
MicroRNAs 10a and 10b are potent inducers of neuroblastoma cell differentiation
through targeting of nuclear receptor corepressor 2.
NH Foley, I Bray, KM Watters, S Das, K Bryan, T Bernas, JHM Prehn and RL Stallings (2011)
Cell Death and Differentiation. 18:1089-98.
PMID: 21212796
Application of Scepter: Cell counting to monitor cell growth (SK-N-BE cells, a human
neuroblastoma cell line)
Bardet–Biedl syndrome highlights the major role of the primary cilium in efficient water
reabsorption.
Vincent Marion, Dominique Schlicht, Anaı¨s Mockel, Sophie Caillard, Olivier Imhoff, Corinne
Stoetzel, Paul van Dijk, Christian Brandt, Bruno Moulin, He´ le`ne Dollfus (2011) International
Society of Nephrology. 79:1013-25.
PMID: 21270763
Application of Scepter: Cell counting, Cell volume distributions measurement (HCD cells, a cell
line generated from kidney cortical collecting duct cells)