高纯度重组β-葡萄糖醛酸酶(大肠杆菌),用于研究,生化酶测定和体外诊断分析。
内容:500,000单位
运输温度:环境
储存温度:2-8oC
配方:溶液(Tris.HCl / NaCl / EDTA)
物理形式:解决方案
稳定性:在4oC下> 4年
酶活性:β-葡萄糖醛酸酶
EC号:3.2.1.31
CAZy家庭:GH2
CAS号:9001-45-0
同义词:β-D-葡萄糖醛酸苷葡萄糖醛酸水解酶;古斯
来源:大肠杆菌
分子量:82,600
浓度:〜250 kU / mL
表达:重组自大肠杆菌
特异性:从糖结合物的糖蛋白和寡糖中水解非还原性末端β-D-葡萄糖醛酸残基。
比活度:〜15,000 U / mg(37°C,酚酞-β-D-葡糖醛酸苷的pH 6.8);
〜50 U / mg(37oC,pNP-β-D-葡糖醛酸苷pH 7.5)
单位定义:30000 U / mg蛋白质:一单位的β-D-葡萄糖醛酸苷酶活性定义为每小时从磷酸钠缓冲液中的酚酞-β-D-葡萄糖醛酸(0.5 mM)中释放出一微克酚酞所需的酶量。 (100 mM)在pH 6.8和37oC下。
110 U / mg蛋白:单位β-D-葡萄糖醛酸苷酶活性定义为在Tris.HCl缓冲液中每分钟从pNP-β-D-葡萄糖醛酸苷(1 mM)释放1 µmol对硝基苯酚所需的酶量。 (410 mM)pH 7.5和37oC。,在410 nm处监测。
温度最佳:37oC
最适pH值:6.8
High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Content: 500,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In solution (Tris.HCl/NaCl/EDTA)
Physical Form: Solution
Stability: > 4 years at 4oC
Enzyme Activity: β-Glucuronidase
EC Number: 3.2.1.31
CAZy Family: GH2
CAS Number: 9001-45-0
Synonyms: β-D-glucuronoside glucuronosohydrolase; GUS
Source: Escherichia coli
Molecular Weight: 82,600
Concentration: Supplied at ~ 250 kU/mL
Expression: Recombinant from Escherichia coli
Specificity: Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.
Specific Activity: ~ 15,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide);
~ 50 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide)
Unit Definition: 30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37oC.
110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37oC., monitored at 410 nm.
Temperature Optima: 37oC
pH Optima: 6.8
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