| 细胞名称 | NK-92人恶性非霍奇金淋巴瘤患者的自然杀伤细胞, |
| 描述 | NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征： CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性，CD56亮；CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。 |
| 动物种别 | 人 |
| 性别 | 男 |
| 组织来源 | 恶性非霍奇金淋巴瘤患者的自然杀伤细胞, |
| 形态 | 淋巴母细胞 |
| 培养基和添加剂 | 添加2mM L-谷氨酰胺，0.2mM 肌醇, 0.1mM β-疏基乙醇，0.02mM 叶酸， 100-200单位/ml重组 IL-2, 含1.5g/L 碳酸钠，不含核糖核酸和脱氧核糖核酸的MEM α，75%；马血清， 12.5%；FBS，12.5%。 |
| 供应限制 | A。仅供研究之用。 |
| REFERENCE | Gong JH , et al. Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells. Leukemia 8: 652-658, 1994. PubMed: 8152260Klingemann HG , et al. A cytotoxic NK-cell line (NK-92) for ex vivo purging of leukemia from blood. Biol. Blood Marrow Transplant. 2: 68-75, 1996. PubMed: 9118301Tam YK , et al. Characterization of genetically altered, interleukin 2-independent natural killer cell lines suitable for adoptive cellular immunotherapy. Hum. Gene Ther. 10: 1359-1373, 1999. PubMed: 10365666Klingemann HG , Miyagawa B . Purging of malignant cells from blood after short ex vivo incubation with NK-92 cells. Blood 87: 4913-1914, 1996. PubMed: 8639869Komatsu F , Kajiwara M . Relation of natural killer cell line NK-92-mediated cytolysis (NK-92-lysis) with the surface markers of major histocompatibility complex class I antigens, adhesion molecules, and Fas of target cells. Oncol. Res. 10: 483-489, 1998. PubMed: 10338151Yan Y , et al. Antileukemia activity of a natural killer cell line against human leukemias. Clin. Cancer Res. 4: 2859-2868, 1998. PubMed: 9829753Maki G , et al. Induction of sensitivity to NK-mediated cytotoxicity by TNF-alpha treatment: possible role of ICAM-3 and CD44. Leukemia 12: 1565-1572, 1998. PubMed: 9766501Nagashima S , et al. Stable transduction of the interleukin-2 gene into human natural killer cell lines and their phenotypic and functional characterization in vitro and in vivo. Blood 91: 3850-3861, 1998. PubMed: 9573023Tam YK , et al. Immunotherapy of malignant melanoma in a SCID mouse model using the highly cytotoxic natural killer cell line NK-92. J. Hematother. 8: 281-290, 1999. PubMed: 10417052 |
| 中文名称 | 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞, |
| 价格 | 1000元 |
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