454 GS Junior系统将增加测序读长及推出新自动化方案

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454 GS Junior测序仪将在2012-2013年增加测序读长及推出新自动化方案

454 GS Junior测序仪最近推出了其Phase B的一系列改进，包括新版本的测序软件v2.7，相比以前v2.5软件，对于图像处理有明显提升，可以带来更加稳定的测序结果，尤其是扩增子的测序。同时，454公司也将在2012年-2013年间陆续推出一系列提高测序性能以及自动化工作流程的GS Junior升级方案。GS Junior系统在传染病，癌症，环境微生物及农业研究方面都有广泛应用。¹

新的测序软件v2.7提高了测序稳定性以及测序效率，除了在图像处理方面的提升外也在GS De Novo Assembler, GS Reference Mapper以及GS Amplicon Variant Analyzer软件上增加了一些新的功能。在实验方面，paired end测序（可以获得跨越3 kb, 8 kb或者20 kb区域的两段序列）推出了使用GS FLX Titanium Rapid Library Preparation Kits的文库制备方案，加快建库速度，同时可以为样本添加特异性标签进行混合测序。

此次特别宣布了关于GS Junior系统的一系列后续升级方案，包括增加测序读长，自动化文库制备和emPCR工作流程的计划。这些方面的提升将帮助原先使用传统Sanger测序方法的研究人员方便转移到454测序平台上，无论是基础研究还是转化医学领域的研究人员，能使用GS Junior系统。这些改进预计最快在今年的晚些时候或者明年年初正式推出。

454 Life Sciences总裁Thomas Schinecker先生说道：“我们很高兴看到GS Junior系统在全球得到推广，同时也很兴奋地向大家宣布我们为不断提高其性能、简便操作所做的各种努力及规划。这些改进将使得454的长度长平台更加人性化，使得更多传统测序使用者可以转移到二代测序平台上。”

¹ 了解更多GS Junior相关文献，请浏览 http:///publications.

Roche Launches Software Upgrade for GS Junior System, Announces Long Read Improvements and Workflow Automation for 2012/2013

Apr 16, 2012 - fuchsc8

Roche announced today the immediate availability of a new software upgrade and further upcoming sequencing performance and workflow improvements to the company’s 454 GS Junior System. Now a widely published benchtop next-generation sequencing platform, the GS Junior System is rapidly advancing research worldwide in areas of infectious disease, cancer, environmental microbiology and agriculture¹.

The new software package (v2.7) improves sequencing consistency and robustness and also includes functionality improvements to the GS De NovoAssembler, GS Reference Mapper, and GS Amplicon Variant Analyzer Software. The company also announced the immediate availability of new protocols for paired end sequencing (3 kb, 8 kb, 20 kb span) using the GS FLX Titanium Rapid Library Preparation Kits. The protocols enable simple sample multiplexing for paired end projects and reduce overall prep time.

In addition, the company has announced upcoming improvements to the GS Junior System, which include extension to sequencing read lengths and automation of the library preparation and emPCR workflow. The improvements will further ease the transition of projects from traditional Sanger capillary sequencing to 454 Sequencing Systems in basic and translational research laboratories around the world. The planned improvements will first become available in late 2012 and during 2013.

“We are pleased by the adoption of the GS Junior System worldwide and are excited to announce our efforts to continuously improve performance and ease of use of the platform,” said Thomas Schinecker, President of 454 Life Sciences, a Roche Company. “These improvements make our long read sequencing solution more user friendly and enable customers to convert projects from Sanger to next-generation sequencing more easily.”

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