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3161 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
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2990 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
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2723 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
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1715 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
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1887 次 Isolation of lymphatic endothelial cells 2012-3-12
Dermal Cell Suspensions1.Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2.Dermal sheets were prepared by incubatio
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5537 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
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3119 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
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2186 次 Placental trophoblast and chorionic cell cultures 2011-12-16
Placental trophoblast and chorionic cell cultures 1.Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2.Term human placentae a
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3815 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut
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5142 次 干细胞3D scaffold培养技术的优势、特色及应用范围 2011-12-5
3D培养的优势： 2D细胞培养时，细胞几乎百分之五十是贴平于培养皿，而另外百分之五十是浸泡在溶液中，最为重要的细胞与细胞间接触却相当的少，使得细胞的生长形态与真实情况差距甚远，易造成不
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1541 次 Isolation of human prostatic epithelial cells 2011-11-25
Isolation of human prostatic epithelial cells1.A small piece of tissue from each specimen was removed and minced. 2.The tissue was digested with collagenase overnight. 3.To remove
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3199 次 Isolation of stromal vascular cells from human adipose tissue 2011-9-30
Isolation of stromal vascular cells from human adipose tissue Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal ste
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3283 次 Isolation and cultivation of endothelial progenitor cells (EPCs) 2011-9-30
Isolation and cultivation of endothelial progenitor cells (EPCs) Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue rege
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1718 次 Isolation of dermal mast cell (DMC) 2011-9-22
Isolation of dermal mast cell (DMC) 1.Human DMC were isolated from skin by enzymatic digestion.2.Dermal tissue was obtained from patients with skin after informed consent was give
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9147 次 Isolation of retinal pigment epithelial cell 2011-9-16
Isolation of retinal pigment epithelial cell The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that
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2376 次 Trabecular cell monolayer culture 2011-9-2
Trabecular cell monolayer cultureOriginally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
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2252 次 Isolation of rodent pancreatic β cells 2011-8-1
Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal
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2269 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla
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2053 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were
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2208 次 Primary brain cell isolation and culture 2011-6-13
Primary brain cell isolation and culture. 1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containin